| 1 |
Introduction to the problem |
Assignment 1 out |
| 2 |
Side-chain review
Sulfur redox chemistry
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| 3 |
The Anfinsen experiments |
Assignment 2 out |
| 4 |
Globular protein structure
Protein interiors
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| 5 |
Using the Protein Database (PDB) |
Assignment 3 out |
| 6 |
Helix-helix packing in globular proteins |
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| 7 |
Beta-sheets
Beta-sheet packing
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| 8 |
Experimental techniques: circular dichroism |
Assignment 4 out |
| 9 |
Thermal denaturation
Coiled coils
Refolding of Tropomyosin
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| 10 |
Fluorescence spectroscopy |
Assignment 5 out |
| 11 |
S-peptide helical folding |
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| 12 |
Detecting partially folded intermediates |
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| 13 |
Prolyl isomerization |
Assignment 6 out |
| 14 |
Cytochrome c refolding pathway |
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| 15 |
2-D NMR techniques |
Assignment 7 out |
| 16 |
Collagen structure and folding in vivo |
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| 17 |
Procollagen folding in vitro |
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| 18 |
Protein calorimetry: BPTI |
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| 19 |
Protein misfolding and aggregation |
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| 20 |
Ribosome channel
Nascent chains
Trigger factor
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Graded problem set due |
| 21 |
Scaffolding proteins in viral shell assembly |
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| 22 |
Amyloid fiber formation in neuro degenerative disease |
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| 23 |
Mid-term exam |
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| 24 |
Chaperonin assisted folding |
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| 25 |
Eukariotic chaperonins |
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| 26 |
Paper topic discussion and choices |
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| 27 |
Membrane protein (rhodopsin) folding and assembly |
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| 28 |
Prion diseases |
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| 29 |
Etiology of some human protein deposition diseases |
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| 30-37 |
Student presentations |
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| 38 |
Final papers due |
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