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Subject Description
This class provides an introduction to the interactions between cells and the surfaces of biomaterials. The course covers: surface chemistry and physics of selected metals, polymers, and ceramics; surface characterization methodology; modification of biomaterials surfaces; quantitative assays of cell behavior in culture; biosensors and microarrays; bulk properties of implants; and acute and chronic response to implanted biomaterials. General topics include biosensors, drug delivery, and tissue engineering.
Prerequisites
8.01; 8.02; 3.091, 5.111, or 5.112; 7.012, 7.013 or 7.014; 3.012, 5.60, or 2.40.
Texts
Ratner, Buddy D., et al. Biomaterials Science: An Introduction to Materials in Medicine. 2nd ed. Burlington, MA: Academic Press, 2004. ISBN: 9780125824637.
Supplemental Handouts.
Homework
Approximately biweekly (7 problem sets).
Tests
Two quizzes and one final exam.
Grading
Grading criteria.
| ACTIVITIES |
PERCENTAGES |
| Homework* |
40% |
| Quiz 1 |
15% |
| Quiz 2 |
15% |
| Final Exam |
30% |
* weighting may vary
Note: Students may discuss homework problems, but should write up solutions independently. Quizzes and final are closed-book exams.
Calendar
Several lectures are given by the course TA Dr. Ikuo Taniguchi.
Course calendar.
| SES # |
TOPICS |
ASSIGNMENTS |
| 1 |
Course Introduction
Biomaterials Defined
Structural Hierarchy in Materials and Biology |
Problem set 1 assigned |
| 2 |
Biomaterials Surfaces: Physics
Surface (vs. Bulk) Structure and Properties
Surface Energy
Adsorption, Segregation, and Reconstruction at Surfaces |
|
| 3 |
Biomaterials Surfaces: Chemistry
Reactions at Surfaces: Chemisorption, Corrosion |
|
| 4 |
Biomaterials Surfaces: Chemistry (cont.)
Hydrolysis (Dr. Taniguchi) |
Problem set 1 due
Problem set 2 assigned |
| 5 |
Protein-Surface Interactions
Proteins: Structure, Properties, Functions
Protein Adsorption: Langmuir Model |
|
| 6 |
Protein-Surface Interactions (cont.)
Protein Adsorption: Complex Phenomena, Measurement |
Problem set 2 due
Problem set 3 assigned |
| 7 |
Cell-Surface Interactions: Host Response to Biomaterials
Cell Adhesion Mechanisms
Coagulation Cascade |
|
| 8 |
Cell-Surface Interactions: Host Response to Biomaterials (cont.)
Immune Response: Alternative Complement Activation |
|
| 9 |
Surface Modification Methods
Purposes
Plasma Treatments
Organic Coatings (Dr. Taniguchi) |
Problem set 3 due |
|
Quiz 1 |
|
| 10 |
Surface Modification Methods (cont.)
Polymer Coatings
Patterned Surfaces |
Problem set 4 assigned |
| 11 |
Surface Characterization
In Vacuum: XPS/ESCA, AES, SIMS |
|
| 12 |
Surface Characterization In situ
Contact Angle, AFM |
Problem set 4 due |
| 13 |
Quantifying Cell Behavior
Cell Cultures
Cellular Assays: Adhesion, Migration, Proliferation |
Problem set 5 assigned |
| 14 |
Quantifying Cell Behavior (cont.)
Cellular Assays: Differentiation, Function |
|
| 15 |
Statistical Analysis
Error Sources, Distribution Functions, t- test |
|
| 16 |
Statistical Analysis (cont.)
Fisher Test, Regression Methods |
Problem set 5 due
Problem set 6 assigned |
| 17 |
Biosensors and Diagnostic Devices
Biological Elements
Transduction Mechanisms
Properties
Examples: Fiber Optic Biosensors, Nanobarcodes |
Problem set 6 due |
|
Quiz 2 |
|
| 18 |
Drug Delivery: Controlled Release (Dr. Taniguchi)
Chemically-controlled Devices |
|
| 19 |
Drug Delivery: Controlled Release (cont.)
Diffusion-controlled, Membrane-based and Osmotic Devices |
Problem set 7 assigned |
| 20 |
Drug Delivery: Controlled Release (cont.)
Methods: Transdermal, Colloidal Vehicles
Mechanical Pumps |
|
| 21 |
Biomaterials for Organ Replacement
Organ Replacement Therapies
Mechanical Properties
Bone Substitutes |
|
| 22 |
Tissue Engineering
Cell Types; Approaches: In vitro, In vivo, Ex vivo/Cell Encapsulation
Scaffolds: Design and Fabrication |
Problem set 7 due |
| 23 |
Tissue Engineering (cont.)
Case Examples: Artificial Pancreas, Cartilage, Nerve Regeneration |
|
| 24 |
FDA Regulatory Issues and Course Evaluations |
|
|
Final Exam |
|