20.109 | Spring 2010 | Undergraduate

Laboratory Fundamentals in Biological Engineering

Lecture Notes

This page presents complete lecture slides for modules 2 and 3, plus several sessions on the 20.109 writing and oral presentation requirements.

SES # MODULE DAY LECTURE TOPICS LECTURE SLIDES
1   Orientation (PDF - 1.7MB)

Module 1: RNA Engineering (Prof. Niles)

The Module 1 lecture notes are not available for OCW, except for the following writing and communications content.

3 2 Writing and communications introduction (Neal Lerner) (PDF)
The Language of Biological Engineering Reports (Linda Sutliff) (PDF)
6 5 Creating your 20.109 presentation (Atissa Banuazizi) (PDF - 1.4MB)
Module 2: Protein Engineering (Prof. Jasanoff)
10 1 Introduction (PDF - 1.6MB)
11 2 Rational protein design (PDF)
12 3 Fluorescence and sensors (PDF - 1.3MB)
13 4 Protein expression (PDF)
14 5 Review & gene analysis (PDF)
15 6 Purification and protein analysis (PDF - 2.0MB)
16 7 Binding & affinity measurements (PDF)
17 8 High throughput engineering (PDF)
Module 3: Cell-Biomaterial Engineering (Dr. Stachowiak)
18 1 Introduction

(PDF - 1.5MB)

Supplemental videos

19 2 Introduction to biomaterials; cartilage composition (PDF - 1.6MB)
20 3 Basic statistics; standards in scientific communities I (PDF - 1.1MB)
21 4 Standards in scientific communities II; cell viability (PDF)
22 5 Assays for transcription and protein levels (PDF)
23 6 Cartilage TE: from in vitro and in vivo models to the clinic (PDF - 1.5MB)
24 7 Creating your proposal presentation (Atissa Banuazizi) (PDF)
25 8 Drug and gene delivery; clinical progress in engineering tissues besides cartilage Chalkboard lecture, no notes available
Learning Resource Types
Other Video
Presentation Assignments
Written Assignments
Lecture Notes