This section provides video tutorials that explore the theory and practice of using the lab AFM, which differs somewhat from standard commercial AFM systems. http://ocw.mit.edu/courses/biological-engineering/20-309-biological-engineering-ii-instrumentation-and-measurement-fall-2006/tools 2013-01-12T01:30:35+05:00 MIT OpenCourseWare http://ocw.mit.edu en-US Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm <p><em>Tutorial 1: Introduction to the lab AFM (40 min.)</em></p><p>Tour of the AFM components</p><p>Hardware operation: preparing and loading the cantilever and sample; setup and alignment using the laser diffraction pattern</p><p>Software operation: overview; interpreting computer and oscilloscope displays</p><p>Force spectroscopy introduction: engaging the tip; monitoring cantilever deflection; fine-tuning the setup with force curves</p>Thumbnail - <a href= http://img.youtube.com/vi/nAPZvX2i7Fw/default.jpg>JPG (YouTube)</a><br>Video - download: <a href= http://www.archive.org/download/MIT20.309F06/ocw-20.309-2007-07-11-tutorial_300k.mp4>Internet Archive (MP4)</a><br>Video - download: <a href= http://itunes.apple.com/us/itunes-u/tutorial-1-introduction-to/id535243793?i=116739007>iTunes U (MP4)</a><br>Video - stream: <a href= http://www.youtube.com/v/nAPZvX2i7Fw>YouTube </a><br><br><a href= 'http://ocw.mit.edu/terms/'>(CC BY-NC-SA)</a><br><br> http://ocw.mit.edu/courses/biological-engineering/20-309-biological-engineering-ii-instrumentation-and-measurement-fall-2006/tools/tutorial-1 Shusteff, Maxim So, Peter Manalis, Scott 2007-04-27T01:55:18+05:00 en-US MIT OpenCourseWare http://ocw.mit.edu Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm <p><em>Hands-on 1: AFM force spectroscopy (15 min.)</em></p><p>Load cantilever and sample; determine basic features of the sample</p>Thumbnail - <a href= http://img.youtube.com/vi/eXusvz0bI4I/default.jpg>JPG (YouTube)</a><br>Video - download: <a href= http://www.archive.org/download/MIT20.309F06/ocw-20.309-2007-07-11-hands-on_300k.mp4>Internet Archive (MP4)</a><br>Video - download: <a href= http://itunes.apple.com/us/itunes-u/hands-on-1-afm-force-spectroscopy/id535243793?i=116739005>iTunes U (MP4)</a><br>Video - stream: <a href= http://www.youtube.com/v/eXusvz0bI4I>YouTube </a><br><br><a href= 'http://ocw.mit.edu/terms/'>(CC BY-NC-SA)</a><br><br> http://ocw.mit.edu/courses/biological-engineering/20-309-biological-engineering-ii-instrumentation-and-measurement-fall-2006/tools/hands-on-1 Shusteff, Maxim So, Peter Manalis, Scott 2007-04-27T01:55:18+05:00 en-US MIT OpenCourseWare http://ocw.mit.edu Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm <p><em>Tutorial 2: More about force spectroscopy (18 min.)</em></p><p>Collecting and analyzing data</p><p>Comparing the 20.309 lab AFM and commercial AFM systems</p><p>Overview of elastic modulus curves experiment</p><p>Cantilever geometry correction factor (numerical calculation of cantilever bending)</p>Thumbnail - <a href= http://img.youtube.com/vi/juTnY6Wad84/default.jpg>JPG (YouTube)</a><br>Video - download: <a href= http://www.archive.org/download/MIT20.309F06/ocw-20.309-2007-07-12-tutorial_300k.mp4>Internet Archive (MP4)</a><br>Video - download: <a href= http://itunes.apple.com/us/itunes-u/tutorial-2-more-about-force/id535243793?i=116739002>iTunes U (MP4)</a><br>Video - stream: <a href= http://www.youtube.com/v/juTnY6Wad84>YouTube </a><br><br><a href= 'http://ocw.mit.edu/terms/'>(CC BY-NC-SA)</a><br><br> http://ocw.mit.edu/courses/biological-engineering/20-309-biological-engineering-ii-instrumentation-and-measurement-fall-2006/tools/tutorial-2 Shusteff, Maxim So, Peter Manalis, Scott 2007-04-27T01:55:18+05:00 en-US MIT OpenCourseWare http://ocw.mit.edu Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm <p><em>Hands-on 2: AFM force spectroscopy (43 min.)</em></p>Thumbnail - <a href= http://img.youtube.com/vi/Tyajd1ZddcQ/default.jpg>JPG (YouTube)</a><br>Video - download: <a href= http://www.archive.org/download/MIT20.309F06/ocw-20.309-2007-07-12-hands-on_300k.mp4>Internet Archive (MP4)</a><br>Video - download: <a href= http://itunes.apple.com/us/itunes-u/hands-on-2-afm-force-spectroscopy/id535243793?i=116739006>iTunes U (MP4)</a><br>Video - stream: <a href= http://www.youtube.com/v/Tyajd1ZddcQ>YouTube </a><br><br><a href= 'http://ocw.mit.edu/terms/'>(CC BY-NC-SA)</a><br><br> http://ocw.mit.edu/courses/biological-engineering/20-309-biological-engineering-ii-instrumentation-and-measurement-fall-2006/tools/hands-on-2 Shusteff, Maxim So, Peter Manalis, Scott 2007-04-27T01:55:18+05:00 en-US MIT OpenCourseWare http://ocw.mit.edu Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm <p><em>Tutorial 3: AFM noise Measurement (16 min.)</em></p><p>Estimating Boltzman's constant</p><p>More about cantilever behavior</p>Thumbnail - <a href= http://img.youtube.com/vi/HS27vd1io8M/default.jpg>JPG (YouTube)</a><br>Video - download: <a href= http://www.archive.org/download/MIT20.309F06/ocw-20.309-2007-07-13-tutorial_300k.mp4>Internet Archive (MP4)</a><br>Video - download: <a href= http://itunes.apple.com/us/itunes-u/tutorial-3-afm-noise-measurement/id535243793?i=116739003>iTunes U (MP4)</a><br>Video - stream: <a href= http://www.youtube.com/v/HS27vd1io8M>YouTube </a><br><br><a href= 'http://ocw.mit.edu/terms/'>(CC BY-NC-SA)</a><br><br> http://ocw.mit.edu/courses/biological-engineering/20-309-biological-engineering-ii-instrumentation-and-measurement-fall-2006/tools/tutorial-3 Shusteff, Maxim So, Peter Manalis, Scott 2007-04-27T01:55:18+05:00 en-US MIT OpenCourseWare http://ocw.mit.edu Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm <p><em>Hands-on 3: AFM noise measurement (9 min.)</em></p><p>More about preparing and loading cantilevers</p>Thumbnail - <a href= http://img.youtube.com/vi/JIjr9CmN5eI/default.jpg>JPG (YouTube)</a><br>Video - download: <a href= http://www.archive.org/download/MIT20.309F06/ocw-20.309-2007-07-13-hands-on_300k.mp4>Internet Archive (MP4)</a><br>Video - download: <a href= http://itunes.apple.com/us/itunes-u/hands-on-3-afm-noise-measurement/id535243793?i=116739004>iTunes U (MP4)</a><br>Video - stream: <a href= http://www.youtube.com/v/JIjr9CmN5eI>YouTube </a><br><br><a href= 'http://ocw.mit.edu/terms/'>(CC BY-NC-SA)</a><br><br> http://ocw.mit.edu/courses/biological-engineering/20-309-biological-engineering-ii-instrumentation-and-measurement-fall-2006/tools/hands-on-3 Shusteff, Maxim So, Peter Manalis, Scott 2007-04-27T01:55:18+05:00 en-US MIT OpenCourseWare http://ocw.mit.edu Content within individual OCW courses is (c) by the individual authors unless otherwise noted. MIT OpenCourseWare materials are licensed by the Massachusetts Institute of Technology under a Creative Commons License (Attribution-NonCommercial-ShareAlike). For further information see http://ocw.mit.edu/terms/index.htm