This session will explain how a collection of mutants with the same phenotype can be used to study a biochemical pathway. In effect, this session outlines how genetics can be used to study biochemistry. It includes a discussion of yeast as a model system. It outlines how to identify mutants with mutations in the same pathway, how to characterize the mutants into complementation groups, and how to use epistasis tests to infer details about a biochemical pathway.
For this session, watch the video lecture called "Genetics 3" by Prof. Eric Lander recorded in 2004. Then watch the first 7 ½ minutes of the lecture called "Human Genetics."
First, watch the short video of Genny Gould explaining the concept of Complementation.
Next, watch Genny work a Complementation Problem (PDF) using fruit flies as an example.
Complementation (Part II)