Agarose Gel Electrophoresis, DNA Sequencing, PCR

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Session Overview

This session will outline methods to analyze genes identified through recombinant DNA technologies. Learning Objectives To understand what an agarose gel is and how to use agarose gel electrophoresis to analyze DNA molecules. To understand the basic mechanism of DNA sequencing by the dideoxy chain termination method. To know that there is a vast database containing the DNA sequence of the entire genomes for many different organism, and understand why this is useful. To understand the basic mechanism of the polymerase chain reaction (PCR) method of amplifying DNA and know when to use this technique.

Session Activities

Lecture Video

Watch the lecture video excerpt

• Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 1 (00:17:46)

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  Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 1

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Session Activities

Lecture Video

Watch the lecture video excerpt

• Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 2 (00:42:26)

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  Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 2

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Check Yourself

Session Activities

Help Session Video

Watch the short video of Robert Dorkin explaining the Polymerase Chain Reaction (PCR) and DNA sequencing.

• Polymerase Chain Reaction (PCR) (00:08:33)

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  Polymerase Chain Reaction (PCR)

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Practice Problems

• Problems (PDF)
• Solutions (PDF)

Further Study

Useful Links

• DNA Sequencing
• Jonathan Weissman: DNA Sequencing
• Biology Animation Library
• PCR Virtual Lab

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