The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
You will be doing real research in this lab. Each research project will center on examining the biology of Pseudomonas aeruginosa. However, each team will be designing their own laboratory experiments to research different aspects of the bacterium. Students will choose from a list of possible projects (PDF).
Your notebook should be a complete record of all your experiments as they were actually performed. A good notebook will enable someone to reconstruct, long after the fact, exactly what was done and why.
Information on the Experimental Microbial Genetics Lab Notebook (PDF)
The following is an index of laboratory protocols that were used by students in this course.
You will be required to archive the strain and plasmids you construct during the semester.
- Guidelines for archiving with strain and plasmid list template (PDF)
- Archive example for plasmid sequence and map (PDF)
- Results from phenazine screen on tryptone plates (PDF)
- List of common primers (XLS)
- pUCP18 sequence (PDF)
- pUCP18 map
- pMQ64 sequence (PDF)
- pMQ64 map (PDF)