| Module 1: Size and Components of Cells and Implications with respect to Regulation |
| L1 |
Introduction: cell constituents, prokaryotes vs. eukaryotes (PDF) |
| L2 |
Introduction (cont'd) (PDF) |
| Module 2: Fatty Acid Synthases (FAS), Polyketide Synthases (PKS), and Non-ribosomal Polypeptide Synthases (NRPS) |
| L3 |
Fatty Acid Synthase: polymerization, biosynthesis, players, chemistry, structure, chemistry as a paradigm for PKS and NRPS, medical interlude (PDF) |
| L4 |
Experimental methods for elucidating FAS structure (PDF) |
| L5 |
Chemistry of FAS as paradigm for other molecular machines (PDF) |
| L6 |
Secondary metabolism: PKS, NRPS (PDF) |
| L7 |
Chemistry of PKS and NRPS: post-translational modification, initiation, elongation, decoration, termination, fidelity (PDF) |
| L8 |
Chemistry of PKS and NRPS (cont'd) (PDF) |
| L9 |
Chemistry of PKS and NRPS (cont'd with specific examples) (PDF) |
| L10 |
Biosynthesis of yersiniabactin and cholesterol (PDF) |
| L11 |
Cholesterol biosynthesis (PDF) |
| L12 |
Cholesterol regulation and homeostasis (PDF) |
| L13 |
Sensing insoluble molecules (PDF) |
| L14 |
Module 2: Regulation of the transcription level by insoluble metabolites and Module 3: Translation (PDF) |
| Module 3: Translation: Loading, Initiation, Elongation, and Termination - A Machine in Action; Introduction to G-proteins: Switches or Motors |
| L15 |
Translation (cont'd) (PDF) |
| L16 |
Elongation, termination, RNA polymerase (PDF) |
| L17 |
Chemical methods for studying translation and the ribosome (PDF) |
| L18 |
Chemical methods for studying translation and the ribosome (cont'd) (PDF) |
| L19 |
Chemical methods for studying translation and the ribosome (cont'd) (PDF) |
| L20 |
Isoleucine tRNA synthetase (PDF) |
| L21 |
tRNA synthase editing mechanisms; G proteins (EF-Tu/EF-G) (PDF) |
| L22 |
G proteins: motors (PDF) |
| L23 |
G proteins: switches (PDF) |
| L24 |
Peptide bond formation; new technologies using the ribosome (PDF) |
| L25 |
Module 3: methods for the incorporation of unnatural amino acids and Module 4: what happens as a protein exits the ribosome? (PDF) |
| Module 4: Crypts and Chambers: Macromolecular Machines involved in Protein Folding and Degradation |
| L26 |
Protein folding in vitro (PDF) |
| L27 |
Protein folding: in vitro vs. in vivo; degradation (PDF) |
| L28 |
Protein folding in vivo (PDF) |
| L29 |
Chaperone proteins (PDF) |
| L30 |
GroEL/GroES (PDF) |
| L31 |
Proteases (PDF) |
| L32 |
Proteosome (PDF) |
| L33 |
Proteosome (cont'd) (PDF) |
| L34 |
Role of Ubiquitin in degradation (PDF) |
| L35 |
Degradation through polyubiquitination (PDF) |
