Biochemistry Laboratory

Gleevec molecule inside purple ribbons of enzyme.

Gleevec bound to the Abl kinase domain. (PDB ID: 1IEP. Nagar, B., W. Bornmann, P. Pellicena, T. Schindler, D. R. Veach, W. T. Miller, B. Clarkson, J. Kuriyan. "Crystal Structures of the Kinase Domain of c-Abl in Complex with the Small Molecule Inhibitors PD173955 and Imatinib." Cancer Research. 62 (2002): 4236-4243. Image by Elizabeth Vogel Taylor.)


MIT Course Number


As Taught In

Spring 2009



Cite This Course

Course Features

Course Description

The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec. Techniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.

This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format.


Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan.

Elizabeth Taylor. 5.36 Biochemistry Laboratory, Spring 2009. (Massachusetts Institute of Technology: MIT OpenCourseWare), (Accessed). License: Creative Commons BY-NC-SA

For more information about using these materials and the Creative Commons license, see our Terms of Use.