PK Sl7L L imsmanifest.xml
IMS Content
1.1
OCW
CWSpace
0.1
Experimental Microbial Genetics
OCW Master Course Number
7.13
Fall 2008
OCW_LOMv1.0
Author
Newman, Dianne
2021-12-11
OCW_LOMv1.0
Author
Melvold, Janis
2021-12-11
OCW_LOMv1.0
Author
Croal, Laura
2021-12-11
OCW_LOMv1.0
Author
Laub, Michael
2021-12-11
OCW Course Topics
Science
Biology
Genetics
OCW Course Topics
Science
Biology
Microbiology
contents/index.htm.xml
Experimental Microbial Genetics
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Experimental Microbial Genetics
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Black and white micrograph of P. aeruginosa.
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Black and white micrograph of P. aeruginosa
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Syllabus
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Calendar
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Molecular Biology Boot Camp
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Bootcamp Day 2 — Purification, digestion and dephosphorylation of pMQ64
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Bootcamp Day 1 — Start a culture of E. coli + pMQ64 and streak Pseudomonas aeruginosa PA14 for single colonies
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Bootcamp Day 6: Pooling colonies and extracting plasmid clone DNA
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Bootcamp Day 5 — Gel Extraction of correctly sized PA14 genomic DNA fragments and BamHI linearized, dephosphorylated pMQ64, Ligation and Transformation
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Boot camp day 7: PCR verification of insert size
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Bootcamp Day 3 — Purification of Genomic DNA from Pseudomonas aeruginosa PA14 using the QIAGEN Genomic-Tip Kit and preparation of agarose gels
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Bootcamp Day 4 — Partial digestion of Pseudomonas aeruginosa PA14 genomic DNA and Gel Electrophoresis
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Labs
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Project List
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Plasmid DNA Purification Using the QIAprep Spin Miniprep Kit and a Microcentrifuge
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Restriction digests
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Dephosphorylating with CIP and Ligation Reactions
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Protocol for spot cfu/ml counts
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pMQ64 map
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pMQ64 sequence
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Transforming DH5a-T1R Chemically Competent Cells
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Results from phenazine screen on tryptone plates
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Plasmid or Cosmid DNA Purification Using HiSpeed Plasmid Midi and Maxi Kits
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CAS Agar for Pseudomonas
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Archiving the tools you make this term
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PCR with the Platinum PCR SuperMix High Fidelity
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Care and Handling of PA01 fur mutants
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Generating and screening for mutants in Fur
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Motility Assays
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pUCP18 sequence
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Agarose Gel Electrophoresis and DNA Band Excision
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QIAquick PCR & Enzyme Purification Kit Protocol
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QIAquick Gel Extraction Kit Protocol
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Biofilm Assay
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Pseudomonas Electroporations
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Digital Optical Microscopy
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UV spectrophotometric measurement of DNA concentration and purity
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pT9
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Characterization of phenazines in Pseudomonas
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List of common primers
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Primer Design Guidelines for PCR Reactions
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Experimental Design and Interpretation, Technical Skill, Quality of Effort, Lab Notebook and Lab Citizenship
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Presentations
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Assignments
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Writing a Primary Research Report (a scientific paper)
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Results & Discussion
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Readings
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Related Resources
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Legal Notices
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Privacy Statement
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Trademark Notices
contents/syllabus/index.htm.xml
contents/molecular-biology-boot-camp/MIT7_13f08_lab31.pdf.xml
contents/labs/MIT7_13f08_lab05_Protocol_CAS.pdf.xml
contents/7-13f08.jpg.xml
contents/7-13f08-th.jpg.xml
contents/labs/MIT7_13f08_lab27_pMQ64_sequence.pdf.xml
contents/labs/Common_Primers.xls.xml
contents/index.htm.xml
contents/calendar/index.htm.xml
contents/labs/MIT7_13f08_lab17_Protocol_QiagenMiniprep.pdf.xml
contents/labs/MIT7_13f08_lab20_Protocol_Restriction.pdf.xml
contents/assignments/MIT7_13f08_assn02_Results.pdf.xml
contents/labs/MIT7_13f08_lab08_Protocol_Designing.pdf.xml
contents/labs/MIT7_13f08_lab24_ArchiveExample.pdf.xml
contents/labs/MIT7_13f08_lab14_Protocol_Plate.pdf.xml
contents/labs/MIT7_13f08_lab13_Protocol_PCR.pdf.xml
contents/labs/MIT7_13f08_lab02_Protocol_Agarose.pdf.xml
contents/labs/MIT7_13f08_lab06_Protocol_Characterization.pdf.xml
contents/assignments/MIT7_13f08_assn01_Intro.pdf.xml
contents/labs/index.htm.xml
contents/labs/MIT7_13f08_lab15_Protocol_Pseudomonas.pdf.xml
contents/labs/MIT7_13f08_lab16_Protocol_QiagenHiSpeed.pdf.xml
contents/labs/MIT7_13f08_lab07_Protocol_Dephosphorylating.pdf.xml
contents/molecular-biology-boot-camp/index.htm.xml
contents/labs/MIT7_13f08_lab26_pPUP18_sequence.pdf.xml
contents/labs/MIT7_13f08_lab11_Protocol_Motility.pdf.xml
contents/assignments/index.htm.xml
contents/molecular-biology-boot-camp/MIT7_13f08_lab30.pdf.xml
contents/labs/MIT7_13f08_lab19_Protocol_QIAquickPCR.pdf.xml
contents/labs/MIT7_13f08_lab03_Protocol_Biofilm.pdf.xml
contents/readings/index.htm.xml
contents/labs/MIT7_13f08_lab28_pMQ64_Map.pdf.xml
contents/labs/MIT7_13f08_lab21_Protocol_Spectrophotometric.pdf.xml
contents/related-resources/index.htm.xml
contents/labs/MIT7_13f08_lab09_Protocol_DIC.pdf.xml
contents/labs/MIT7_13f08_lab10_Protocol_Generating.pdf.xml
contents/labs/MIT7_13f08_lab04_Protocol_Care.pdf.xml
contents/labs/MIT7_13f08_lab22_Protocol_Transformation.pdf.xml
contents/molecular-biology-boot-camp/MIT7_13f08_lab32.pdf.xml
contents/molecular-biology-boot-camp/MIT7_13f08_lab33.pdf.xml
contents/labs/MIT7_13f08_lab18_Protocol_QIAquickGel.pdf.xml
contents/molecular-biology-boot-camp/MIT7_13f08_lab29.pdf.xml
contents/labs/MIT7_13f08_lab01_ProjectSummaries.pdf.xml
contents/labs/MIT7_13f08_lab25_PhenazineScreen.pdf.xml
contents/molecular-biology-boot-camp/MIT7_13f08_lab34.pdf.xml
contents/labs/MIT7_13f08_res01.pdf.xml
contents/labs/MIT7_13f08_lab23_ArchiveGuide.pdf.xml
contents/presentations/index.htm.xml
contents/molecular-biology-boot-camp/MIT7_13f08_lab35.pdf.xml
PK S~3! 7-13-fall-2008/ReadMe.txtThis zip package contains the HTML pages and files associated with the course.
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If you have any trouble using this package, please contact us at ocw@mit.edu .PK S%aD6 6 7-13-fall-2008/START.htm
MIT OpenCourseWare | Welcome
Welcome to MIT Open Course Ware. You will be automatically redirected to Home. If you aren not forwarded to the new page, Click here to access the home page of the downloaded Click Here
PK S>P7 P7 ' 7-13-fall-2008/contents/7-13f08.jpg.xml
Black and white micrograph of P. aeruginosa.
Scanning Electron Micrograph of Pseudomonas aeruginosa bacteria. (Image courtesy of Janice Haney Carr, Centers for Disease Control and Prevention.)
en
LOMv1.0
2
Fall 2008
OCW_LOMv1.0
Author
Newman, Dianne
2021-12-11
OCW_LOMv1.0
Author
Melvold, Janis
2021-12-11
OCW_LOMv1.0
Author
Croal, Laura
2021-12-11
OCW_LOMv1.0
Author
Laub, Michael
2021-12-11
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This site (c) Massachusetts Institute of Technology 2021. Content within individual courses is (c) by the individual authors unless otherwise noted. The Massachusetts Institute of Technology is providing this Work (as defined below) under the terms of this Creative Commons public license ("CCPL" or "license") unless otherwise noted. The Work is protected by copyright and/or other applicable law. Any use of the work other than as authorized under this license is prohibited. By exercising any of the rights to the Work provided here, You (as defined below) accept and agree to be bound by the terms of this license. The Licensor, the Massachusetts Institute of Technology, grants You the rights contained here in consideration of Your acceptance of such terms and conditions.
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OCW Master Course Number
7.13 Experimental Microbial Genetics Fall 2008
In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production.
Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized.
WARNING NOTICE
The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
Legal Notice
CIP
260502
Microbiology, General
microbiology
genetics
pseudomonas
bacteria
genes
pathogen
mutagenesis
PCR
DNA sequencing
enzyme assays
gene expression
molecular genetics
plasmid manipulation
genetic complementation
laboratory
protocol
vector
mutant
cystic fibrosis
PK S]:4 :4 % 7-13-fall-2008/contents/index.htm.xml
Experimental Microbial Genetics
In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production.
Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized.
WARNING NOTICE
The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
Legal Notice
OCW Master Course Number
7.13
en
LOMv1.0
4
Fall 2008
OCW_LOMv1.0
Author
Newman, Dianne
2021-12-11
OCW_LOMv1.0
Author
Melvold, Janis
2021-12-11
OCW_LOMv1.0
Author
Croal, Laura
2021-12-11
OCW_LOMv1.0
Author
Laub, Michael
2021-12-11
2009/12/10 16:00:22 US/Eastern
2018/04/17 04:56:57.953 GMT-4
/courses/biology/7-13-experimental-microbial-genetics-fall-2008/index.htm
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LOMv1.0
Browser
LOMv1.0
Internet Explorer
9.0
LOMv1.0
Browser
LOMv1.0
Edge
13.1
OCW_LOMv1.0
Undergraduate
LOMv1.0
yes
This site (c) Massachusetts Institute of Technology 2021. Content within individual courses is (c) by the individual authors unless otherwise noted. The Massachusetts Institute of Technology is providing this Work (as defined below) under the terms of this Creative Commons public license ("CCPL" or "license") unless otherwise noted. The Work is protected by copyright and/or other applicable law. Any use of the work other than as authorized under this license is prohibited. By exercising any of the rights to the Work provided here, You (as defined below) accept and agree to be bound by the terms of this license. The Licensor, the Massachusetts Institute of Technology, grants You the rights contained here in consideration of Your acceptance of such terms and conditions.
CIP
260502
Microbiology, General
microbiology
genetics
pseudomonas
bacteria
genes
pathogen
mutagenesis
PCR
DNA sequencing
enzyme assays
gene expression
molecular genetics
plasmid manipulation
genetic complementation
laboratory
protocol
vector
mutant
cystic fibrosis
PK Sh+Sl$' $' &