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7.002 | Spring 2025 | Undergraduate

# Fundamentals of Experimental Molecular Biology

The manual contains the experiments and background information for the course and details the procedures to be completed during each day in the lab. The experiments in this course are divided into 10 days spent in the lab, followed by extra material in the appendix.

7.002 Lab Manual (PDF)

Lab Manual Sections | Pages
--- | ---
Day 1 | 5–16
Day 2 | 17–20 ; 21–27
Day 3 | 28–30 ; 31–38
Day 4 | 39–43
Day 5 | 44–51
Day 6 | 52–60
Day 6.5 | 61–62
Day 7 | 63–75
Day 8 | 76–85
Day 9 | 86–92
Day 10 | 93–102
APPENDIX
Lab Safety Guidelines | 104–107
Eppendorf BioSpectrometer Instructions | 108–109
Molecular Weight Markers and Amino Acid Structures | 110
Codon Chart | 111
Primers Available for RTH Mutagenesis | 112
ClpXdeltaN Coding Sequence | 113–114
pET Vector Cloning Site Sequence | 115
Plasmid Maps and Strain Info | 116

Note: In the on-campus course, students completed their pre-labs on an internal 7.002 MITx site, which is not represented here. They interactively learned the background of lab techniques and answered some questions to ensure they understood the lab manual and experimental setup before coming to lab.

## Course Info

##### Instructors

- Dr. Eric Chu
- Dr. Vanessa Cheung

##### Departments

- Biology

##### As Taught In

Spring 2025

##### Level

Undergraduate

##### Topics

- Science
  - Biology
    - Cell Biology
    - Genetics
    - Molecular Biology

##### Learning Resource Types

assignment Activity Assignments
assignment Laboratory Assignments
assignment Written Assignments

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