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7.002 | Spring 2025 | Undergraduate

# Fundamentals of Experimental Molecular Biology

#### Lab Day 6

- 3.1: Analysis of sequencing data
- 3.2: Transformation of E. coli LOBSTR BL21(DE3) with mutant clpX plasmids
- 3.3: Make purification buffers.

#### Lab Day 6.5

- 3.4: Expression of ClpX mutant protein and collection of cell pellet

*** Note: Part 6.5 will be performed by the 7.002 teaching staff

#### Lab Day 7

- 3.5: Analysis of E. coli LOBSTR BL21(DE3) transformations
- 3.6: Purification of mutant ClpX ATPase protein
- 3.7: Determination of purified mutant ClpX protein concentration

## Course Info

##### Instructors

- Dr. Eric Chu
- Dr. Vanessa Cheung

##### Departments

- Biology

##### As Taught In

Spring 2025

##### Level

Undergraduate

##### Topics

- Science
  - Biology
    - Cell Biology
    - Genetics
    - Molecular Biology

##### Learning Resource Types

assignment Activity Assignments
assignment Laboratory Assignments
assignment Written Assignments

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