# Module 1, Day 7: Results

These selected lab results are courtesy of the students and used with permission. (T/R blue = Leanna Morinishi and an anonymous MIT student; all other teams anonymous MIT students.)

GROUP Corrected A405 values Pre % 8-12:
expected, measured
Post %  8-12:
fewer washes, more washes (measured)
Syntax Sample 0.1, 0.2, 0.3
0.4, 0.5, 0.6
10%, 12%  40%, 50%

We used Grey group's 6-5 because we didn't have any.

T/R Orange 0.1814, 0.182, 0.2368
0.1835, 0.2560, 0.2815
2%, 3.3696% 4 washes: 134.8675%;
24 washes: 181.1195%

Only used 1nmols each for the binding assay samples.
Peak shifts are kind of as expected. 8-12 and post mixtures have peaks at a higher wavelength than 6-5 and heme, as expected. However, post mixtures have higher absorbance relative to 8-12 pre absorbance at peaks than expected.

W/F Yellow 0.2623, 0.2580, 0.3333
0.2620, 0.2895, 0.2863
2%, 5.4% 8 Washes 41.8%;
16 washes 37.6%

During the dilution step, we accidentally diluted it to 7.7uM as opposed to the 8uM. (This is estimating final concentration to be 50% 8-12). However, we corrected this by decreasing the amount of heme so that it was still a 1:1 ratio. Therefore, the results should not have been affected. Normalization was used to correct for the heme curve, which was lower than all the other curves. Enrichment values were close to expected ones (50%) but slightly surprising that 8 washes enriched a bit more than 16 washes.

T/R Green 0.244, 0.242, 0.319
0.252, 0.286, 0.312
10%, 12.987% 4 washes: 57.143%;
24 washes: 90.909%

Due to the corrections, the heme and 6-5 aptamer horizontal peaks are a little off; the 6-5 aptamer graph should be shifted up from the heme graph at low absorbances, but instead, it's simply lined up with the heme graph. The other graphs seem consistent with the fact that 8-12 graph should be higher horizontally than the 6-5 graph, with the heights increasing in the following order: mix 10% pre, mix 10% post 4x, post 24x, and 8-12 aptamer.

W/F Red 0.260, 0.252, 0.323
0.262, 0.311, 0.30888
10%, 14% 82.5%
80.9%

T/R
Blue
0.261, 0.263, 0.322
0.294, 0.3111, 0.306
50%, 52.2905% 8 washes: 80.3992%
16 washes: 71.7277%