1 00:00:00,000 --> 00:00:08,070 2 00:00:08,070 --> 00:00:08,600 PROFESSOR: Hi. 3 00:00:08,600 --> 00:00:10,670 In this clip, we're going to discuss the numbering and 4 00:00:10,670 --> 00:00:15,730 labeling conventions of RNA and of protein. 5 00:00:15,730 --> 00:00:20,270 Here we have a nucleoside triphosphate or NTP. 6 00:00:20,270 --> 00:00:24,110 This NTP is numbered on each carbon. 7 00:00:24,110 --> 00:00:28,180 And we're going to start here to the right of this oxygen 8 00:00:28,180 --> 00:00:33,580 and label one prime and proceed two prime, three 9 00:00:33,580 --> 00:00:40,820 prime, four prime, and this last, five prime carbon. 10 00:00:40,820 --> 00:00:45,560 This means that when we polymerize two or more of 11 00:00:45,560 --> 00:00:50,630 these NTP's to get an RNA chain, it becomes apparent why 12 00:00:50,630 --> 00:00:53,770 one end is labeled five prime and the other end is labeled 13 00:00:53,770 --> 00:00:55,160 three prime. 14 00:00:55,160 --> 00:01:00,200 On this end, the extending atom is the five prime carbon. 15 00:01:00,200 --> 00:01:04,269 And on this side, the other end, the extending atom is the 16 00:01:04,269 --> 00:01:08,040 three prime carbon attached to this oxygen. 17 00:01:08,040 --> 00:01:11,120 This three prime carbon is where you would attach another 18 00:01:11,120 --> 00:01:14,340 NTP if you would wish to extend this chain. 19 00:01:14,340 --> 00:01:19,070 And so we label the ends of RNA five prime to three prime. 20 00:01:19,070 --> 00:01:21,600 And RNA is always polymerized in the five prime to the three 21 00:01:21,600 --> 00:01:22,850 prime direction. 22 00:01:22,850 --> 00:01:26,170 23 00:01:26,170 --> 00:01:31,150 Now with our amino acid, we label this end, which is our 24 00:01:31,150 --> 00:01:36,690 amino terminus, as N and this end which is our carboxy 25 00:01:36,690 --> 00:01:43,740 terminus as C. As before, when we polymerize three amino 26 00:01:43,740 --> 00:01:47,880 acids into this short protein chain, I'm going to label this 27 00:01:47,880 --> 00:01:53,660 end which has a protruding amino terminus as N and this 28 00:01:53,660 --> 00:02:00,370 end which has a protruding carboxy terminus as C. 29 00:02:00,370 --> 00:02:04,260 So that's how the conventions are in science to label and 30 00:02:04,260 --> 00:02:06,540 number amino acids and proteins. 31 00:02:06,540 --> 00:02:07,790 Thanks for watching. 32 00:02:07,790 --> 00:02:08,405