1 Introduction  
2 G-protein coupled receptors and rhodopsin

Unger, V. M., P. A. Hargrave, J. M. Baldwin, and G. F. X. Schertler. "Arrangement of Rhodopsin Transmembrane α-helices." Nature 389 (1997): 203-206.

Palczewski, K., T. Kumasaka, T. Hori, C. A. Behnke, H. Motoshima, B. A. Fox, I. L. Trong, D. C. Teller, T. Okada, R. E. Stenkamp, M. Yamamoto, and M. Miyano. "Crystal Structure of Rhodopsin: A G Protein-Coupled Receptor." Science 289 (2000): 739-745.

3 Visual cascade part I - Activation of rhodopsin by light

Zhukovsky, E. A., and D. D. Oprian. "Effect of Carboxylic Acid Side Chains on the Absorption Maximum of Visual Pigments." Science 246 (1989): 928-930.

Farrens, D. L., C. Altenbach, K. Yang, W. L. Hubbell, and H. G. Khorana. "Requirement of Rigid-body Motion of Transmembrane Helices for Light Activation of Rhodopsin." Science 274 (1996): 768-770.

4 Visual cascade part II - Rhodopsin and G protein (transducin) interaction

Weiss, E. R., D. J. Kelleher, and G. L. Johnson. "Mapping Sites of Interaction between Rhodopsin and Transducin Using Rhodopsin Antipeptide Antibodies." J Biol Chem 263 (1988): 6150-6154.

Natochin, M., M. Moussaif, and N. O. Artemyev. "Probing Mechanism of Rhodopsin-catalyzed Transducin Activation." J Neurochem 77 (2001): 202-210.

5 Visit to an Atomic Force Microscopy Facility

Fotiadis, D., S. Scheuring, S. A. Müller, A. Engel, and D. J. Müller. "Imaging and Manipulation of Biological Structures with the AFM." Micron 33 (2002): 385-397.

Field trip schedule (PDF)

6 Rhodopsin dimerization

Fotiadis, D., Y. Liang, S. Filipek, D. A. Saperstein, A. Engel, and K. Palczewski. "Rhodopsin Dimers in Native Disc Membranes." Nature 421 (2003): 127-128.

Liang, Y., D. Fotiadis, S. Filipek, D. A. Saperstein, K. Palczewski, and A. Engel. "Organization of the G Protein Coupled Receptors Rhodopsin and Opsin in Native Membranes." J Biol Chem 278 (2003): 21655-21662.

7 Rhodopsin mutations, retinal degeneration and night blindness

Sung, C. H., B. G. Schneider, N. Agarwal, D. S. Papermaster, and J. Nathans. "Functional Heterogeneity of Mutant Rhodopsins Responsible for Autosomal Dominant Retinitis Pigmentosa." Proc Natl Acad Sci 88 (1991): 8840-8844.

Jin, S., M. C. Cornwall, and D. D. Oprian. "Opsin Activation as a Cause of Congenital Night Blindness." Nature Neuroscience 6 (2003): 731-735.

Pulse-Chase experiment

8 Field trip to the Novartis Institutes for BioMedical Research  
9 Drug addiction - Dopamine receptors and activation mechanism

Lee, S. P., C. H. So, A. J. Rashid, G. Varghese, R. Cheng, A. J. Lanca, B. F. O'Dowd, and S. R. George. "Dopamine D1 and D2 Receptor Co-activation Generates a Novel Phospholipase C-mediated Calcium Signal." J Biol Chem 279 (2004): 35671-35678.

Guo, W., L. Shi, M. Filizola, H. Weinstein, and J. A. Javitch. "Crosstalk in G Protein-coupled Receptors: Changes at the Transmembrane Homodimer Interface Determine Activation." Proc Natl Acad Sci 102 (2005): 17495-17500.

Dopamine receptors

Molecular mechanism of drug addiction

D1/D2 synergism

10 Allergies - Histamine receptors

Morisset, S., A. Rouleau, X. Ligneau, F. Gbahou, J. Tardivel-Lacombe, H. Stark, W. Schunack, C. R. Ganellin, Schwartz, and J-M. Arrang. "High Constitutive Activity of Native H3 Receptors Regulates Histamine Neurons in Brain." Nature 408 (2000): 860-864.

Gillard, M., C. Van Der Perren, N. Moguilevsky, R. Massingham, and P. Chatelain. "Binding Characteristics of Cetirizine and Levocetirizine to Human H1 Receptors: Contribution of Lys191 and Thr194." Mol Pharmacol 61 (2002): 391-399.

Milligan, G., R. A. Bond, and M. Lee. "Inverse Agonism: Pharmacological Curiosity or Potential Therapeutic Strategy?" Trends in Pharmacological Sciences 16, no. 1 (January 1995): 10-13.

11 How do mutations in chemokine receptors inhibit CCR5-mediated HIV infection?

Benkirane, M., D-Y Jin, R. F. Chun, R. A. Koup, and K-T Jeang. "Mechanism of Transdominant Inhibition of CCR5-mediated HIV-1 Infection by ccr5d32." J Biol Chem 272 (1997): 30603-30606.

Hernanz-Falcon, P., J. M. Rodriguez-Frade, A. Serrano, D. Juan, A. D. Sol, S. F. Soriano, F. Roncal, L. Gomez, A. Valencia, C. Martinez-A, and M. Mellado. "Identification of Amino Acid Residues Crucial for Chemokine Receptor Dimerization." Nature Immunology 5 (2004): 216-223.

Supplementary material for Hernanz-Falcon, et al

Flow cytometry

Yeast two hyrbid system

12 Sense of smell: Olfactory receptors

Buck, L., and R. Axel. "A Novel Multigene Family May Encode Odorant Receptors: Molecular Basis for Odor Recognition." Cell 65 (1991): 175-187.

Oka, Y., M. Omura, H. Kataoka, and K. Touhara. "Olfactory Receptor Antagonism between Odorants." EMBO J 23 (2004): 120-126.

Techniques used in the papers:

13 Sense of taste: Taste receptors

Nelson, G., M. A. Hoon, J. Chandrashekar, Y. Zhang, N. J. P. Ryba, and C. S. Zuker. "Mammalian Sweet Taste Receptors." Cell 106 (2001): 381-390.

Moon, S. J., M. Köttgen, Y. Jiao, H. Xu, and C. Montell. "Taste Receptor Required for the Caffeine Response in Vivo." Current Biology 16 (2006): 1812-1817.

———. "Supplemental Data: Taste Receptor Required for the Caffeine Response in Vivo." Current Biology 16 (2006): 1812-1817.

14 Oral presentations: General discussion and future perspectives