For each reading listed below, each student will be expected to be able to provide explanations to a set of questions handed out at the start of the course (PDF)

1 Introduction to bacterial stress response  
2 Bleaching in cyanobacteria

Schwarz, R., and A. R. Grossman. "A Response Regulator of Cyanobacteria Integrates Diverse Environmental Signals and is Critical for Survival under Extreme Conditions." Proc Natl Acad Sci 95 (1998): 11008-11013.

Aldehni, M. F., J. Sauer, C. Spielhaupter, R. Schmid, and K. Forchhammer. "Signal Transduction Protein P(II) is required for NtcA-regulated Gene Expression during Nitrogen Deprivation in the Cyanobacterium Synechococcus Elongatus Strain PCC 7942." J Bacteriol 185 (2003): 2582-2591.

3 Down in the deep blue: thriving in deep-sea vents

Beatty, J. T., J. Overmann, M. T. Lince, A. K. Manske, A. S. Lang, R. E. Blankenship, C. L. Van Dover, T. A. Martinson, F. G. Plumley. "An Obligately Photosynthetic Bacterial Anaerobe from a Deep-sea Hydrothermal Vent." Proc Natl Acad Sci 102 (2005): 9306-9310.

Nakagawa, S., Y. Takaki, S. Shimamura, A. L. Reysenbach, K. Takai, and K. Horikoshi. "Deep-sea Vent Epsilon-proteobacterial Genomes provide Insights into Emergence of Pathogens." Proc Natl Acad Sci 121 (2007): 12146-12150.

4 Cleaning up the Earth: bacteria and toxic chromium pollutants

Ackerley, D. F., Y. Barak, S. V. Lynch, J. Curtin, and A. Matin. "Effect of Chromate Stress on Escherichia Coli K-12." J Bacteriol 188 (2006): 3371-3381.

Chourey, K., M. R. Thompson, J. Morrell-Falvey, N. C. Verberkmoes, S. D. Brown, M. Shah, J. Zhou, M. Doktycz, R. L. Hettich, and D. K. Thompson. "Global Molecular and Morphological Effects of 24-hour Chromium (VI) Exposure on Shewanella Oneidensis MR-1." Appl Environ Microbiol 72 (2006): 6331-6344.

5 Plants as pals in survival

Davies, B. W., and G. C. Walker. "A Highly Conserved Protein of Unknown Function is Required by Sinorhizobium Meliloti for Symbiosis and Environmental Stress Protection." J Bacteriol 190 (2008): 1118-1123.

Gourion, B., A. Francez-Charlot, and J. A. Vorholt. "PhyR is Involved in the General Stress Response of Methylobacterium Extorquens AM1." J Bacteriol 190 (2008): 1027-1035.

6 What's for dinner? Stress responses and food

Price, S. B., J. C. Wright, F. J. DeGraves, M. P. Castanie-Cornet, and J. W. Foster. "Acid Resistance Systems Required for Survival of Escherichia Coli O157:H7 in the Bovine Gastrointestinal Tract and in Apple Cider are Different." Appl Environ Microbiol 70 (2004): 4792-4799.

Hüfner, E., T. Markieton, S. Chaillou, A. M. Crutz-Le Coq, M. Zagorec, and C. Hertel. "Identification of Lactobacillus Sakei Genes Induced during Meat Fermentation and their Role in Survival and Growth." Appl Environ Microbiol 73 (2007): 2522-2531.

7 Sticking together: biofilms

Shrout, J. D., D. L. Chopp, C. L. Just, M. Hentzer, M. Givskov, and M. R. Parsek. "The Impact of Quorum Sensing and Swarming Motility on Pseudomonas Aeruginosa Biofilm formation is Nutritionally Conditional." Mol Microbiol 62 (2006): 1264-1277.

Viswanathan, P., M. Singer, and L. Kroos. "Role of Sigma-D in Regulating Genes and Signals during Myxococcus Xanthus Development." J Bacteriol 188 (2006): 3246-3256.

8 Combating reactive oxygen species and establishing infection

Wang, G., Y. Hong, A. Olczak, S. E. Maier, and R. J. Maier. "Dual Roles of Helicobacter pylori NapA in Inducing and Combating Oxidative Stress." Infect Immun 74 (2006): 6839-6846.

De Groote, M. A., U. A. Ochsner, M. U. Shiloh, C. Nathan, J. M. McCord, M. C. Dinauer, S. J. Libby, A. Vazquez-Torres, Y. Xu, and F. C. Fang. "Periplasmic Superoxide Dismutase Protects Salmonella from Products of Phagocyte NADPH-oxidase and Nitric Oxide Synthase." Proc Natl Acad Sci 94 (1997): 13997-14001.

9 When viruses attack: phage shock response

Brissette, J. L., M. Russel, L. Weiner, and P. Model. "Phage Shock Protein, a Stress Protein of Escherichia Coli." Proc Natl Acad Sci 87 (1990): 862-866.

Jovanovic, G., L. J. Lloyd, M. P. Stumpf, A. J. Mayhew, and M. Buck. "Induction and Function of the Phage Shock Protein Extracytoplasmic Stress Response in Escherichia Coli." J Biol Chem 281 (2006): 21147-21161.

10 Tug-of-war for Iron: bacterial mechanisms for evading host innate immunity and other bacterial species in order to acquire iron

Fischbach, M. A., H. Lin, L. Zhou, Y. Yu, R. J. Abergel, D. R. Liu, K. N. Raymond, B. L. Wanner, R. K. Strong, C. T. Walsh, A. Aderem, and K. D. Smith. "The Pathogen-associated iroA gene Cluster Mediates Bacterial Evasion of Lipocalin 2." Proc Natl Acad Sci 103 (2006): 16502-16507.

Semenova, E., Y. Yuzenkova, J. Peduzzi, S. Rebuffat, and K. Severinov. "Structure-activity Analysis of MicrocinJ25: Distinct Parts of the Threaded Lasso Molecule are Responsible for Interaction with Bacterial RNA Polymerase." J Bacteriol 187 (2005): 3859-3863.

11 Living well in freezing weather

Muryoi, N., M. Sato, S. Kaneko, K. Kawahara, H. Obata, M. W. Yaish, M. Griffith, B. R. Glick. "Cloning and Expression of afpA, a Gene Encoding an Antifreeze Protein from the Arctic Plant Growth-promoting Rhizobacterium Pseudomonas Putida GR12-2." J Bacteriol 186 (2004): 5661-5671.

Yoshimune, K., A. Galkin, L. Kulakova, T. Yoshimura, N. Esaki. "Cold-active DnaK of an Antarctic Psychrotroph Shewanella sp. Ac10 Supporting the Growth of DnaK-null Mutant of Escherichia Coli at Cold Temperatures." Extremophiles 9 (2005): 145-150.

12 Raman spectroscopy for evaluation of bacterial survival

Wu, Q., W. H. Nelson, S. Elliot, J. F. Sperry, M. Feld, R. Dasari, and R. Manoharan. "Intensities of E Coli Nucleic Acid Raman Spectra Excited Selectively from Whole Cells with 251-nm Light." Anal Chem 72 (2000): 2981-2986.

Jorge Villar, S. E., H. G. M. Edwards, and L. G. Benning. "Raman Spectroscopic and Scanning Electron Microscopic Analysis of a Novel Biological Colonisation of Volcanic Rocks." Icarus 184 (2006): 158-169.

13 Various stress responses of Deinococcus

Makarova, K. S., et al. "Deinococcus Geothermalis: The Pool of Extreme Radiation Resistance Genes Shrinks." PLoS ONE 2 (2007): e955.

Liu, Y., et al. "Transcriptome Dynamics of Deinococcus Radiodurans Recovering from Ionizing Radiation." Proc Natl Acad Sci 100 (2003): 4191-4196.

14 Oral presentations