1 00:00:04,350 --> 00:00:04,890 Hi everyone. 2 00:00:04,890 --> 00:00:08,290 Welcome to the first week in our series of classes. 3 00:00:08,290 --> 00:00:11,270 In this tutorial, we're going to take a look 4 00:00:11,270 --> 00:00:15,410 at how you can use a flatbed scanner, believe it or not, 5 00:00:15,410 --> 00:00:19,710 to create some very fine images of three-dimensional objects, 6 00:00:19,710 --> 00:00:23,920 like microfluidic devices, Petri dishes, and other sorts 7 00:00:23,920 --> 00:00:28,180 of material you're creating or working with in the lab. 8 00:00:28,180 --> 00:00:30,050 Now you might be wondering why we're 9 00:00:30,050 --> 00:00:33,100 starting this course with a tool that you probably 10 00:00:33,100 --> 00:00:34,930 wouldn't think of for creating really 11 00:00:34,930 --> 00:00:36,900 fine images of your work. 12 00:00:36,900 --> 00:00:42,250 Well, we want you to experience the fun part of creating images 13 00:00:42,250 --> 00:00:44,680 at first, and frankly, we wanted you 14 00:00:44,680 --> 00:00:49,180 to start off with some easy stuff-- well, relatively easy-- 15 00:00:49,180 --> 00:00:52,980 and not having to worry too much about technical issues, which 16 00:00:52,980 --> 00:00:55,390 believe me, will come later. 17 00:00:55,390 --> 00:00:57,490 That's a promise. 18 00:00:57,490 --> 00:00:59,110 This course is meant to encourage 19 00:00:59,110 --> 00:01:02,230 you to capture your material in a number of ways. 20 00:01:02,230 --> 00:01:08,210 And probably most important, to learn to see in the process. 21 00:01:08,210 --> 00:01:11,100 So you're seeing, and will continue to see, 22 00:01:11,100 --> 00:01:14,410 all sorts of images that I made on the flatbed scanner 23 00:01:14,410 --> 00:01:16,220 with different approaches. 24 00:01:16,220 --> 00:01:20,070 I'll go into detail for a few of them in all of these tutorials 25 00:01:20,070 --> 00:01:22,930 and we'll call them case studies. 26 00:01:22,930 --> 00:01:25,620 You'll see things that will surprise you 27 00:01:25,620 --> 00:01:28,240 and you'll discover new perspectives, that 28 00:01:28,240 --> 00:01:30,690 is new points of view, which might 29 00:01:30,690 --> 00:01:33,300 lead to new thinking about your work. 30 00:01:33,300 --> 00:01:35,650 Try out your ideas about imaging. 31 00:01:35,650 --> 00:01:36,840 It won't take long. 32 00:01:36,840 --> 00:01:39,350 That's the nice part about the flatbed scanner. 33 00:01:39,350 --> 00:01:42,910 Some of your ideas will work, and some won't. 34 00:01:42,910 --> 00:01:46,940 Believe me, a lot of them won't, from my personal experience. 35 00:01:46,940 --> 00:01:49,900 And emphasizing what I said in the general overview, 36 00:01:49,900 --> 00:01:52,770 most of this is about experimenting, 37 00:01:52,770 --> 00:01:54,720 or in a way playing. 38 00:01:54,720 --> 00:01:57,920 The process, I hope, will become an active discovery, 39 00:01:57,920 --> 00:02:00,410 as it did for me when I made these images. 40 00:02:00,410 --> 00:02:03,150 You should think of this process in a similar way 41 00:02:03,150 --> 00:02:06,180 as you do with your own investigations. 42 00:02:06,180 --> 00:02:10,650 So using the scanner can, if you choose, come close to imaging 43 00:02:10,650 --> 00:02:13,890 with a microscope, if you make sure you capture your image 44 00:02:13,890 --> 00:02:15,580 at a high enough resolution. 45 00:02:15,580 --> 00:02:18,530 You can start zooming in to your image on your screen 46 00:02:18,530 --> 00:02:21,200 and see things that you could not see with your eyes. 47 00:02:21,200 --> 00:02:24,240 And I'll show you that a little more later on. 48 00:02:24,240 --> 00:02:27,000 One thing-- I wanted to let you know that I digitally 49 00:02:27,000 --> 00:02:30,050 cleaned some of these images so that your eye wouldn't be 50 00:02:30,050 --> 00:02:31,850 distracted with dust particles. 51 00:02:31,850 --> 00:02:33,970 That's important for you to know. 52 00:02:33,970 --> 00:02:38,240 The decision of whether to use a flatbed scanner is yours. 53 00:02:38,240 --> 00:02:41,510 First think of the size of your device or material. 54 00:02:41,510 --> 00:02:44,079 I'd say a safe bet is, if you're planning 55 00:02:44,079 --> 00:02:47,370 to see details from a few millimeters up 56 00:02:47,370 --> 00:02:49,570 to a few centimeters. 57 00:02:49,570 --> 00:02:51,550 But as you'll see in a few moments, 58 00:02:51,550 --> 00:02:56,770 you'll be able to capture detail in the 30 to 50 micron range. 59 00:02:56,770 --> 00:02:58,970 What you'll see and show in your image 60 00:02:58,970 --> 00:03:02,300 will depend on how you set the resolution in the scanner 61 00:03:02,300 --> 00:03:02,970 setting. 62 00:03:02,970 --> 00:03:06,250 For the time being, just think about it this way-- the higher 63 00:03:06,250 --> 00:03:11,290 your DPI setting, that is dots per inch, the more information 64 00:03:11,290 --> 00:03:12,940 you're sending to the sensor. 65 00:03:12,940 --> 00:03:15,260 The more information you're sending, 66 00:03:15,260 --> 00:03:18,690 the more detail you'll be seeing and capturing. 67 00:03:18,690 --> 00:03:22,110 You'll find all about this in our HOW-TO-DO-IT tutorial. 68 00:03:22,110 --> 00:03:25,810 Take a look at that after you've completed this week's tutorial. 69 00:03:25,810 --> 00:03:29,810 You'll see how changing the DPI settings, as we are seeing here 70 00:03:29,810 --> 00:03:33,630 in these three images, will have a direct effect on capturing 71 00:03:33,630 --> 00:03:34,130 details. 72 00:03:36,910 --> 00:03:38,900 Why use it instead of a camera? 73 00:03:38,900 --> 00:03:42,050 Well first of all, you can very quickly 74 00:03:42,050 --> 00:03:44,440 make some very fine images without going 75 00:03:44,440 --> 00:03:46,970 to more complicated setup with your camera, 76 00:03:46,970 --> 00:03:49,930 as you'll see later in the next few weeks. 77 00:03:49,930 --> 00:03:53,360 You can show evidence that you've made something 78 00:03:53,360 --> 00:03:55,340 or that you're observing something. 79 00:03:55,340 --> 00:03:57,760 You might want to show a part of something 80 00:03:57,760 --> 00:04:00,630 larger, a small structure for example. 81 00:04:00,630 --> 00:04:03,740 You'll be able to share some observations quickly 82 00:04:03,740 --> 00:04:04,950 with your colleagues. 83 00:04:04,950 --> 00:04:07,340 You can consider it as a draft image 84 00:04:07,340 --> 00:04:10,880 that you will eventually use in a figure for a submission. 85 00:04:10,880 --> 00:04:14,940 Perhaps you can even use the image as a patent submission. 86 00:04:14,940 --> 00:04:18,579 That's something to think about. 87 00:04:18,579 --> 00:04:20,829 The wonderful surprise here is that you 88 00:04:20,829 --> 00:04:24,380 can put a three-dimensional object on the scanner 89 00:04:24,380 --> 00:04:28,270 and create images that suggest their three-dimensionality. 90 00:04:28,270 --> 00:04:30,760 Yes, you will lose depth of field, 91 00:04:30,760 --> 00:04:33,480 which we'll discuss in the next tutorial about cameras 92 00:04:33,480 --> 00:04:34,240 and lenses. 93 00:04:34,240 --> 00:04:37,500 But still, eventually you'll create sometimes 94 00:04:37,500 --> 00:04:39,900 a knock-your-socksoff image. 95 00:04:39,900 --> 00:04:43,680 Hopefully you're interested in that sort of thing.