10.442 | Spring 2005 | Undergraduate

Biochemical Engineering

Readings

Required Text

[S&K] Shuler, Michael L., and Fikret Kargi. Bioprocess Engineering: Basic Concepts. 2nd ed. Upper Saddle River, NJ: Prentice Hall PTR, 2001. ISBN: 9780130819086.

Additional Resources

Blanch, Harvey W., and D. S. Clark, eds. Biochemical Engineering. New York, NY: Marcel Dekker Incorporated, 1997. ISBN: 9780824700997.

Bailey, James E., and David F. Ollis. Biochemical Engineering Fundamentals. New York, NY: McGraw-Hill Education, 1986. ISBN: 9780070666016.

Rozzell, J. D. “Commercial scale biocatalysis: myths and realities.” Bioorg Med Chem 7, no. 10 (October 1999): 2253-61.

SES # TOPICS READINGS
1 Course Overview, Enzyme Classification S&K. Chapter 1.
2 Enzyme Kinetics (Single-substrate Reactions), Measurement S&K. Chapter 3.1-3.3.3.
3 Enzyme Inhibition S&K. Chapter 3.3.4.
4 Enzyme Inhibition, pH and Temp Effects S&K. Chapter 3.3.5.
5 Non-ideal Enzyme Kinetics Class notes
6 Immobilized Enzyme Kinetics - External S&K. Chapter 3.4.
7 Immobilized Enzyme Kinetics - Internal S&K. Chapter 3.4.
8 Applications of Enzyme Catalysis (Biocatalysis) S&K. Chapter 3.5, 3.6.

Handouts

9 Microbial Growth S&K. Chapter 6.1-6.2.
10 Microbial Growth - Batch Cultivation Profiles S&K. Chapter 6.1-6.2.
11 Stoichiometry of Growth, Product Formation S&K. Chapter 7.
12 Stoichiometry of Growth, Product Formation (cont.) S&K. Chapter 7.
13 Models of Microbial Growth S&K. Chapter 6.3.
14 Models of Microbial Growth (cont.) S&K. Chapter 6.3.
15 Continuous Culture S&K. Chapter 6.4.
16 Continuous Culture (cont.) S&K. Chapter 6.4.
17 Bioreactor Design S&K. Chapter 9.1-9.3.
18 Bioreactor Design (cont.) S&K. Chapter 9.1-9.3.
19 Bioreactor Design (cont.)

20 Mass Transfer S&K. Chapter 6.2.3-6.2.4, 10.2.3.
21 Mass Transfer (cont.) S&K. Chapter 6.2.3-6.2.4, 10.2.3.
22 Heat Transfer, Media and Air Sterilization S&K. Chapter 10.4.
23 Scale-up S&K. Chapter 10.2.4.
24 Scale-down S&K. Chapter 10.2.5.
25 Animal Cell Culture S&K. Chapter 12.
26 Animal Cell Culture (cont.) S&K. Chapter 12.
27 Mixed Cultures S&K. Chapter 16.
28 Mixed Cultures (cont.) S&K. Chapter 16.
29 Medium Development Handouts
30 Medium Development (cont.) Handouts
31 Cellular Engineering - An Engineer’s View Towards Recombinant Organisms S&K. Chapter 14.

Handouts

32 Cellular Engineering/Molecular Biology for Process Engineering S&K. Chapter 14.

Handouts

33 Cellular Engineering/Molecular Biology for Process Engineering (cont.) S&K. Chapter 14.

Handouts

34 Enzyme Engineering for Process Improvement Handouts
35 Enzyme Engineering for Process Improvement (cont.) Handouts

Course Info

Departments
As Taught In
Spring 2005
Learning Resource Types
Problem Sets