Integrated Chemical Engineering Topics I: Introduction to Biocatalysis

Lecture Notes

lec #	TOPICS	lecture notes
1	Course Overview, Enzyme Classification	(PDF)
2	Enzyme Catalysts: Review of Protein Chemistry, Enzyme Specificity	(PDF)
3-4	Lecture 3 Non-kinetic Evaluation of Enzyme Performance, Review of Enzyme Kinetics Lecture 4 Enzyme Kinetics (cont.), pH and Temperature Effects, Enzyme Stability	(PDF)
5-6	Lecture 5  Enzyme Inhibition and Toxicity Lecture 6  Enzyme Inhibition and Toxicity (cont.)	(PDF 1) (PDF 2) (PDF 3)
7	Whole Cell Catalysts: When/Why, Cell Growth vs. Conversion
8	Control of Protein Expression (Growth/Non-growth Associated, Catabolite Repression) Toxicity and Inhibition
9-11	Lecture 9 Engineering Solutions: Acidic/Basic Products, Exothermic Reactions, Enzyme Instability, Equilibrium Reactions Lecture 10 Substrate Solubility, Toxicity Lecture 11 Product Solubility, Toxicity Yield Improvement of Resolutions	(PDF)
12-14	Lecture 12 Process Design for Biocatalytic Processes: Purified Enzymes Lecture 13 Process Design (cont.): Whole Cells Lecture 14 Screening for New Catalysts	(PDF)
15-17	Lecture 15 Improving Catalyst Performance (Biological Solutions): Review of Molecular Biology Lecture 16 Cloning from Whole Cell Catalysts Lecture 17 Site-directed Mutagenesis Directed Evolution of Enzymes
18	Presentations of Design/Development Reports