This page presents complete lecture slides for modules 2 and 3, plus several sessions on the 20.109 writing and oral presentation requirements.
| SES # | MODULE DAY | LECTURE TOPICS | LECTURE SLIDES | |
|---|---|---|---|---|
| 1 | Orientation | (PDF - 1.7MB) | ||
|
Module 1: RNA Engineering (Prof. Niles) The Module 1 lecture notes are not available for OCW, except for the following writing and communications content. |
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| 3 | 2 | Writing and communications introduction (Neal Lerner) | (PDF) | |
| The Language of Biological Engineering Reports (Linda Sutliff) | (PDF) | |||
| 6 | 5 | Creating your 20.109 presentation (Atissa Banuazizi) | (PDF - 1.4MB) | |
| Module 2: Protein Engineering (Prof. Jasanoff) | ||||
| 10 | 1 | Introduction | (PDF - 1.6MB) | |
| 11 | 2 | Rational protein design | (PDF) | |
| 12 | 3 | Fluorescence and sensors | (PDF - 1.3MB) | |
| 13 | 4 | Protein expression | (PDF) | |
| 14 | 5 | Review & gene analysis | (PDF) | |
| 15 | 6 | Purification and protein analysis | (PDF - 2.0MB) | |
| 16 | 7 | Binding & affinity measurements | (PDF) | |
| 17 | 8 | High throughput engineering | (PDF) | |
| Module 3: Cell-Biomaterial Engineering (Dr. Stachowiak) | ||||
| 18 | 1 | Introduction | ||
| 19 | 2 | Introduction to biomaterials; cartilage composition | (PDF - 1.6MB) | |
| 20 | 3 | Basic statistics; standards in scientific communities I | (PDF - 1.1MB) | |
| 21 | 4 | Standards in scientific communities II; cell viability | (PDF) | |
| 22 | 5 | Assays for transcription and protein levels | (PDF) | |
| 23 | 6 | Cartilage TE: from in vitro and in vivo models to the clinic | (PDF - 1.5MB) | |
| 24 | 7 | Creating your proposal presentation (Atissa Banuazizi) | (PDF) | |
| 25 | 8 | Drug and gene delivery; clinical progress in engineering tissues besides cartilage | Chalkboard lecture, no notes available | |
