20.109 | Spring 2010 | Undergraduate

Laboratory Fundamentals in Biological Engineering

Module 1, Day 1: Amplify Aptamer-Encoding DNA

PCR schematic

Description:

Depicted are two complementary strands of DNA, with a desired target fragment shown in green. Primers that can select the target sequence are shown as short arrows, with the dotted lines indicating the extension step of PCR. Note that in the first couple rounds of PCR, products longer than the desired target will be made (dotted lines keep extending). However, these early products themselves become templates that produce the correct product in abundance.

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