Protein Folding and Human Disease

Calendar

SES #	TOPICS	KEY DATES
1	Introduction to Class Introduction to Class; Objectives, Format, Schedule, Student Introductions Features of the Universe of known Protein Sequences and Structures The Anfinsen Experiments
2	Protein Conformation Review Fibrous Proteins Pauling-corey α-helix Helix / helix Packing in Globular Proteins; Helix / Helix Packing in Coiled Coils Amino Acid Sequence Control of Helicity; Helix Start and Stop Signals	Assignment 1 due
3	β-sheet Conformations β-sheet Conformations Sheet / Sheet Packing in Parallel β-sheets In Orthogonal β-sheets β-helices	Assignment 2 due
4	Critical Features of in Vitro Refolding Reactions Failure of Refolding and Off-pathway Aggregation Industrial Scale Refolding in the Biotechnology Industry	Assignment 3 due
5	Structure and Function of Double Barrel Chaperonins I. Prokaryotic GroE / L, Archael Mm-Cpn, and Eukaryotic TRiC Temperature Sensitive Folding Mutants	Assignment 4 due
6	Amyloid Fibers and Neurodegenerative Disease In-Class Guest Lecturer: Prof. Peter Lansbury, Harvard Medical School, “Synuclein as a Therapeutic Target for Parkinson’s Disease: Twenty Years and Counting.”	Assignment 5 due
7	Collagen Folding and Assembly Collagen Triple Helix Forces that Stabilize Collagen-based Birth Defects	Assignment 6 due
8	Protein Deposition Diseases: Light Chain Amyloidosis, Alzheimers, Parkinson’s, Huntington’s, ALS and Others In-Class Guest Lecturer: Dr. Kelly Knee, Pfizer, Cambridge, “Sickle Cell Hemoglobin.”	Assignment 7 due
9	Prions and Functional Amyloids
10	Membrane Proteins Domain-swapping, Loop-sheet Insertion, and Other Pathways for Protein Aggregation / Polymerization
11	Chaperones Ribosome Tunnel, Trigger Factor, HSP 40, HSP 70 and HSP 90 Chaperones ER Chaperones Proteasome and Degradation Pathways
12	Student Paper Presentations Introduction to Class; Objectives, Format, Schedule, Student Introductions ALS DNAK / Trigger Factor
13	Student Paper Presentations Light Chain Amyloidosis Parkinsons
14	Student Paper Presentations Alzheimers	Final Papers due