Cell and Molecular Neurobiology

Readings

Textbook

Levitan, Irwin B., and Leonard K. Kaczmarek. The Neuron: Cell & Molecular Biology. 3rd ed. New York, NY: Oxford University Press, 2001. ISBN: 9780195145236.

List of optional texts (PDF)

Calendar of Reading Assignments

Readings for this course include readings from the class textbook as well as from a variety of peer-reviewed journals.

LEC #	TOPICS	READINGS	RECOMMENDED REVIEWS/READINGS
1	Ion channels I	Chapters 5 and 7
2	Ion channels II	Chapters 5 and 7	Choe, Senyon. “Potassium Channel Structures.” Neuroscience 3 (February 2002): 115-121. Grottesi, Alessandro, Zara A. Sands, and Mark S. P. Sansom. “Potassium Channels: Complete and Undistorted.” Current Biology 15, no. 18 (September 20, 2005): R771-R774.
3	Receptors I	Chapters 11 and 12	Lefkowitz, Robert J. “Historical Review: A Brief History and Personal Retrospective of Seven-transmembrane Receptors.” TRENDS in Pharmacological Sciences 25, no. 8 (August 2004): 413-422. Strader, Catherine D., Tung Ming Fong, Michael R. Tota, and Underwood Dennis. “Structure and Function of G Protein-coupled Receptors.” Annu Rev Biochem 63 (1994): 101-32. Gainetdinov, Raul R., Richard T. Premont, Laura M. Bohn, et al. “Desensitization of G Protein–coupled Receptors and Neuronal Functions.” Annu Rev Neurosci 27 (2004):107-44. Clapham, David E. “TRP Channels as Cellular Sensors.” Nature 426, no. 4 (December 2003): 517-524. Ashmore, Jonathan. “Channel at the Hair’s End.” Nature 432, no. 9 (December 2004): 685-686. Barritt, Greg Barritt, and Grigori Rychkov. “TRPs as Mechanosensitive Channels.” Nature Cell Biology 7, no. 2 (February 2005): 105-107. Corey, David P. “What is the Hair Cell Transduction Channel?” J Physiol 576, no. 1 (2006): 23-28.
4	Receptors II	Chapters 11 and 12	Lefkowitz, Robert J, and Erin J Whalen. “β-Arrestins: Traffic Cops of Cell Signaling.” Current Opinion in Cell Biology 16 (2004): 162-168. Shenoy, Sudha K., Patricia H. McDonald, Trudy A. Kohout, et al. “Regulation of Receptor Fate by Ubiquitination of Activated β2 -Adrenergic Receptor and β-Arrestin.” Science 294, no. 9 (November 2001): 1307-1313. Gilman, Alfred G. “G Proteins and Regulation of Adenylyl Cyclase.” Bioscience Reports 15, no. 2 (April 1995): 65-97. Neer, Eva J. “Heterotrimeric G Proteins: Review Organizers of Transmembrane Signals.” Cell 80 (January 27, 1995): 249-257. Pierce, Kristen L., Richard T. Premont, and Robert J Lefkowitz. “Seven-Transmembrane Receptors.” Molecular Cell Biology 3 (September 2002): 639-650. von Zastrow, Mark, et al. “Regulated Endocytosis of Opioid Receptors: Cellular Mechanisms and Proposed Roles in Physiological Adaptation to Opiate Drugs.” Current Opinion in Neurobiology 13 (2003): 348-353.
5	Introduction; basics of electrophysiology	Chapters 1 and 4	Nowak L., P. Bregestovski, and P. Ascher. “Magnesium Gates Glutamate-activated Channels in Mouse Central Neurons.” Nature 307 (February 1984): 462-465.
6	Modulation of receptor activity	Chapter 13, pp. 315-331
7	Protein targeting, signaling complexes
8	Neuronal polarity / subcellular specialization	Chapter 2	Jiang, Hui, Guo, Wei, Liang, Xinhua, et al. “Both the Establishment and the Maintenance of Neuronal Polarity Require Active Mechanisms: Critical Roles of GSK-3β and Its Upstream Regulators.” Cell 120 (January 14, 2005): 123-135. Ye, Bing, et al. “Growing Dendrites and Axons Differ in Their Reliance on the Secretory Pathway.” Cell 130 (August 24, 2007): 717-729.
9	Repertoire of neuron interactions	Chapters 2, 3, 8, and 16, pp. 395-406
10	Neurogenesis	Chapter 15	Buchman, Joshua J., and Li-Huei Tsai. “Spindle Regulation in Neural Precursors of Flies and Mammals.” Neuroscience 8 (February 2007): 89-100. Ayala, Ramsés, Tianzhi Shu, and Li-Huei Tsai. “Trekking across the Brain: The Journey of Neuronal Migration.” Cell 128 (January 12, 2007): 29-43.
11	Presynaptic mechanisms	Chapter 9	Jahn, Reinhard, Thorsten Lang, and Thomas C. Sudhof. “Membrane Fusion.” Cell 112 (February 21, 2003): 519-533. Rizo, Josep, and Thomas C. Sudhof. “Snares and Munc18 in Synaptic Vesicle Fusion.” Neuroscience 3 (August 2002): 641-653. Edwards, Robert H. “The Neurotransmitter Cycle and Quantal Size.” Neuron 55 (September 20, 2007): 835-858. Galli, Thierry, and Volker Haucke. “Cycling of Synaptic Vesicles: How Far? How Fast!” Sci STKE, no. 88 (June 26, 2001): 1-10. Stevens, Charles F. “Neurotransmitter Release at Central Synapses.” Neuron 40 (October 9, 2003): 381-388. Yoshihara, Motojiro, Bill Adolfsen, and Troy J. Littleton. “Is Synaptotagmin the Calcium Sensor?” Current Opinion in Neurobiology 13 (2003): 315-323. Koh, Tong-Wey Koh, and Hugo J. Bellen. “Synaptotagmin I, a Ca2+ sensor for Neurotransmitter Release.” TRENDS in Neurosciences 26, no. 8 (August 2003): 413-422.
	Mid-term exam
12	Molecular manipulation of neural circuits		Tervo, Dougal, and Alla Y. Karpova. “Rapidly Inducible, Genetically Targeted Inactivation of Neural and Synaptic Activity In vivo.” Current Opinion in Neurobiology 17 (2007): 581-586.
13	Axon guidance		Tessier-Lavigne, Marc, and Corey S. Goodman. “The Molecular Biology of Axon Guidance.” Science 274, no. 5290 (November 15, 1996): 1123-1133.
14	Synapse formation/elimination	Chapter 18
15	Trophic factors	Chapter 16, pp. 408-427	Chao, Moses V. “Neurotrophins and their Receptors: A Convergence Point for many Signaling Pathways.” Neuroscience 4 (April 2003): 299-309.
16	Postsynaptic mechanisms; LTP/LTD	Chapter 20, pp. 558-570	Sheng, Morgan, et al. “Postsynaptic Signaling and Plasticity Mechanisms.” Science 298 (October 25, 2002): 776-780. Merrill, Michelle A., Yucui Chen, Stefan Strack, et al. “Activity-driven Postsynaptic Translocation of CaMKII.” TRENDS in Pharmacological Sciences 26, no. 12 (December 2005): 645-653. Malenka, Robert C., and Mark F. Bear. “LTP and LTD: An Embarrassment of Riches.” Neuron 44 (September 30, 2004): 5-21. Malenka, Robert C. “The Long-term Potential of LTP.” Neuroscience 4 (November 2003): 923-926. Renger, John J., Christophe Egles, and Guosong Liu. “A Developmental Switch in Neurotransmitter Flux Enhances Synaptic Efficacy by Affecting AMPA Receptor Activation.” Neuron 29 (February 2001): 469-484. Kelleher, Raymond J., Arvind Govindarajan, and Susumu Tonegawa. “Translational Regulatory Mechanisms in Persistent Forms of Synaptic Plasticity.” Neuron 44 (September 30, 2004): 59-73. Frey, Uwe, and Richard G. M. Morris. “Synaptic Tagging: Implications for Late Maintenance of Hippocampal Long-term Potentiation.” TINS 21, no. 5 (1998): 181-188. Martin, Kelsey C., and Kenneth S. Kosik. “Synaptic Tagging - Who’s it?” Neuroscience 3 (October 2002): 813-820.
17	Waves and chemoaffinity		Wilkinson, David G. “Multiple Roles of EPH Receptors and Eprins in Neural Development.” Neuroscience 2 (March 2001): 155-164. Feldheim, David A., Young-In Kim, and Andrew D. Bergeman, et al. “Genetic Analysis of Ephrin-A2 and Ephrin-A5 Shows Their Requirement in Multiple Aspects of Retinocollicular Mapping.” Neuron 25 (March 2000): 563-574. Klein, Rüdiger. “Excitatory Eph Receptors and Adhesive Ephrin Ligands.” Current Opinion in Cell Biology 13 (2001): 196-203. Simon, David K., and Dennis D. M. O’Leary. “Development of Topographic Order in the Mammalian Retinocollicular Projection.” The Journal of Neuroscience 12, no. 4 (April 1992): 1212-1232. Simon, David K., Glen T. Pruskyt, Dennis D. M. O’Leary, et al. “N-Methyl-D-Aspartate Receptor Antagonists Disrupt the Formation of a Mammalian Neural Map.” Proc Natl Acad Sci 89 (November 1992): 10593-10597. Zhou, Jimmy Z. “Direct Participation of Starburst Amacrine Cells in Spontaneous Rhythmic Activities in the Developing Mammalian Retina.” The Journal of Neuroscience 18, no. 11 (June 1, 1998): 4155-4165. Butts, Daniel A. “Retinal Waves: Implications for Synaptic Learning Rules during Development.” The Neuroscientist 8, no. 3 (2002): 243-253. Butts, Daniel A., and Daniel S. Rokhsar. “The Information Content of Spontaneous Retinal Waves.” The Journal of Neuroscience 21, no. 3 (February 1, 2001): 961-973. Söhl, Goran, Stephan Maxeiner, and Klaus Willecke. “Expression and Functions of Neuronal Gap Junctions.” Neuroscience 6 (March 2005): 191-200. Torborg, Christine L, Kristi A Hansen, and Marla B Feller. “High Frequency, Synchronized Bursting Drives Eye-specific Segregation of Retinogeniculate Projections.” Nature Neuroscience 8, no. 1 (January 2005): 72-78. Mann, Fanny, William A. Harris, and Christine E. Holt. “New Views on Retinal Axon Development: A Navigation Guide.” Int J Dev Biol 48 (2004): 957-964.
18	Two faces of GABA		Owens, David F., and Arnold R. Kriegstein. “Is There More to Gaba than Synaptic Inhibition?” Neuroscience 3 (September 2002): 715-727.
19	Hodgkin and Huxley (lecture 1)		Hodgkin, A. L., A. F. Huxley, and B. Katz. “Measurement of Current-voltage Relations in the Membrane of the Giant Axon of Loligo.” J Physiol 116 (1952): 424-448. Hodgkin, A. L., and A. F. Huxley. “Currents Carried by Sodium and Potassium Ions through the Membrane of the Giant Axon of Loligo.” J Physiol 116 (1952): 449-472. ———. “The Components of Membrane Conductance in the Giant Axon of Loligo.” J Physiol 116 (1952): 473-496. ———. “The Dual Effect of Membrane Potential on Sodium Conductance in the Giant Axon of Loligo.” J Physiol 116 (1952): 497-506. ———. “A Quantitative Description of Membrane Current and Its Application to Conduction and Excitation in Nerve.” J Physiol 117 (1952): 500-544.
20	Hodgkin and Huxley (lecture 2)	Chapters 19 and 20
21	Aplysia learning		Bailey, Craig H., Eric R. Kandel, and Kausik Si. “The Persistence of Long-term Memory: A Molecular Approach to Self-sustaining Changes in Learning-induced Synaptic Growth.” Neuron 44 (September 30, 2004): 49-57.
22	Drosophila learning		Keene, Alex C., and Scott Waddell. “Drosophila Olfactory Memory: Single Genes to Complex Neural Circuits.” Neuroscience 8 (May 2007): 341-354.
23	Drosophila circadian rhythms		Nitabach, Michael N., and Paul H. Taghert. “Organization of the Drosophila Circadian Control Circuit.” Current Biology 18, no. 2 (January 22, 2008): R84-R93.
24	Epilepsy		Steinlein, Ortrud K. “Genetic Mechanisms that Underlie Epilepsy.” Neuroscience 5 (May 2004): 400-408.
	Final exam