9.12 | Fall 2006 | Undergraduate

Experimental Molecular Neurobiology

Labs

Lab Safety (PDF)

WEEK # LAB TOPICS PROTOCOLS
2 Basic Skills in a Molecular Biology lab (PDF 1) (PDF 2) (PDF)
3 Sequence Analysis and Vector Design (PDF - 1.6 MB) (PDF)
4 PCR and Digestion (PDF) (PDF)
5 DNA Purification and Subcloning  
7 Transformation, Colony Pick-up, Miniprep, and Digestion (PDF) (PDF)
8 Basic Cell Culture (PDF)
9 Primary Culture of Neurons from the Brain Results from Last Session: GFP (JPG 1) (JPG 2), RFP (JPG 1) (JPG 2)
10 SDS-Polyacrylamide Gel Electrophoresis and Western Blotting (PDF) (PDF)
11 Western Blotting 2: Detection (PDF)
12 Brain Tissue Processing (PDF) (PDF)

Course Info

As Taught In
Fall 2006
Learning Resource Types
Presentation Assignments
Written Assignments