The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
This section lists the course’s experiment assignments and accompanying materials.
|EXP #||TITLES||DESCRIPTIONS||# OF LAB PERIODS||LAB MANUALS|
In the course of this synthetic sequence, the student will encounter operations such as sublimation, distillation, and execution of reaction under an inert atmosphere, all common techniques in synthetic chemistry. The principal aims of this experiment are:
|4||Ferrocene Lab Manual (PDF)|
|2||Ellen Swallow Richards Lab||
This is an integrated experiment, which combines techniques from organic, biological, physical, food, and analytical chemistry. Its purpose is to introduce students to Ellen Swallow Richards.
This experiment will also improve students’ lab and computational techniques in the following areas:
|5||Ellen Swallow Richards Lab Manual (PDF)|
|3||Essential Oils Lab||
In this experiment, students will be working with oils prepared from caraway seeds and spearmint leaves. Each oil has a distinct and characteristic odor, yet carvone is the major component in both oils!
Students will be given a sample of either caraway oil or spearmint oil and will separate the carvone from the higher-boiling and lower-boiling impurities (such as limonene), via vacuum distillation.
Next, students will use gas chromatography and refractometry to check the purity of the distillate and to estimate the relative concentrations of limonene and carvone in the oil, and then convert the carvone to its semicarbazone for use in a polarimetric analysis.
After obtaining infrared spectra of the carvone and limonene fractions and interpreting the results, students will characterize the semicarbazone by melting point determination.
Finally, students will visit the X-Ray Crystallography laboratory where spectra of selected crystals will be determined.
|4||Essential Oils Lab Manual (PDF)|
|4||Fischer Esterification Lab||
This is an integrated experiment, which combines techniques from organic, thermodynamics, physical, and analytical chemistry. It has been designed to introduce the student to:
Bruker NMR Automation use. By John H. Grimes, MIT Department of Chemistry Instrumentation Facility.
|5||Catalase Kinetics Lab||
In this experiment, the student will investigate the enzyme activity of catalase by studying the decomposition of H2O2 to form water and oxygen.
Using an oxygen based pressure sensor the student measures the amount of oxygen produced and then calculates the rate of the enzyme catalyzed reaction under various conditions.
The student also completes a protein assay on an unknown sample of catalase using the Coomassie® Plus Protein Assay from Pierce to determine the protein concentration of the sample. The correlation between the actual and calculated concentration gives an indication of the experimental skills used in carrying out the experiment.
|4||Catalase Kinetics Lab Manual (PDF)|
Required Videos from the MIT Digital Laboratory Techniques Manual
In addition to the reading and written preparation for each experiment, students are also assigned to view selected videos from the MIT Digital Laboratory Techniques Manual, a series of videos designed to help students prepare for chemistry laboratory class.
|Volumetric Techniques||Ellen Swallow Richards, Esterification, and Catalase|
|Titration||Ellen Swallow Richards|
|TLC Basics||Ferrocene and Esterification|
|Reaction Work-up I: Extracting, Washing and Drying||Esterification|
|Filtration||Ferrocene, Ellen Swallow Richards, and Essential Oils|
|Using a Balance||Ferrocene, Ellen Swallow Richards, Essential Oils, Esterification, and Catalase|
|Melting Point Determination||Ferrocene and Essential Oils|
|Using an Automatic Pipet||Catalase|
|Making Buffers and Using a pH Meter||Catalase|
|Distillation I||Essential Oils and Esterification|
|Distillation II||Essential Oils|