7.13 | Fall 2008 | Undergraduate
Experimental Microbial Genetics

Readings

Relevant Literature

The following journal articles provide background for the research you will be doing in lab.

Banin, E., M. L. Vasil, and E. P. Greenberg. “Iron and Pseudomonas Aeruginosa Biofilm Formation.” PNAS 102, no. 31 (2005): 11076-81.

Barton, H. A., Z. Johnson, C. D. Cox, A. I. Vasil, and M. L. Vasil. “Ferric Uptake Regulator Mutants of Pseudomonas Aeruginosa with Distinct Alterations in the Iron-Dependent Repression of Exotoxin A and Siderophores in Aerobic and Microaerobic Environments.” Molecular Microbiology 21, no. 5 (1996): 1001-17.

Cherepanov, P. P., and W. Wackernagel. “Gene Disruption in Escherichia Coli: TcR and KmR Cassettes with the Option of Flp-Catalyzed Excision of the Antibiotic-Resistance Determinant.” Gene 158 (1005): 9-14.

Choi, K., and H. P. Schweizer. “Mini-Tn7 Insertion in Bacteria with Single _att_Tn7 Sites: Example Pseudomonas Aeruginosa.” Nature Protocols 1, no. 1 (2006): 153-61.

De Vos, D., M. de Chial, C. Cochez, S. Jansen, B. Tümmler, J. Meyer, and P. Cornelis. “Study of Pyoverdine Type and Production by Pseudomonas Aeruginosa Isolated from Cystic Fibrosis Patients: Prevalence of Type II Pyoverdine Isolates and Accumulation of Pyoverdine-Negative Mutations.” Arch Microbiol 175 (2001): 384-388.

Dietrich, L. E. P., A. Price-Whelan, A. Petersen, M. Whiteley, and D. K Newman. “The Phenazine Pyocyanin is a Terminal Signaling Factor in the Quorum Sensing Network of Pseudomonas Aeruginosa.” Molecular Microbiology 61, no. 5 (2006): 1308-1321.

Dietrich, L. E. P., T. K. Teal, A. Price-Whelan, D. K. Newman. “Redox-Active Antibiotics Control Gene Expression and Community Behavior in Divergent Bacteria.” Science 321 (2008): 1203-6.

Fajardo, A., et al. “The Neglected Intrinsic Resistome of Bacterial Pathogens.” PLoS ONE 3, no. 2 (2008): e1619.

Hantke, K. “Selection Procedure for Deregulated Iron Transport Mutants (fur) in Escherichia Coli K 12: fur not Only Affects Iron Metabolism.” Mol Gen Genet 210 (1987): 135-139.

Hoang, T. T., A. J. Kutchma, A. Becher, and H. P. Schweizer. “Integration-Proficient Plasmids for Pseudomonas Aeruginosa: Site-Specific Integration and Use for Engineering of Reporter and Expression Strains.” Plasmid 43 (2000): 59-72.

Hobbs, M., E. S. R. Collie, P. D. Free, S. P. Livingston, and J. S. Mattick. “PilS and PilR, a Two-Component Transcriptional Regulartory System Controlling Expression of Type 4 Fimbriae in Pseudomonas Aeruginosa.” Molecular Microbiology 7, no. 5 (1993): 669-82.

Köhler, T., L. K. Curty, F. Barja, C. van Delden, and J. Pechére. “Swarming of Pseudomonas Aeruginosa Is Dependent on Cell-to-Cell Signaling and Requires Flagella and Pili.” J Bacteriol 182, no. 21 (2000): 5990-96. (PDF - 1.8MB)

Link, A. J., D. Phillips, and G. M. Church. “Methods for Generating Precise Deletions and Insertions in the Genome of Wild-Type Escherichia coli: Application to Open Reading Fram Characterization.” J Bacteriol 179, no. 20 (1997): 6228-6237.

Masduki, A., J. Nakamura, T. Ohga, R. Umezaki, J. Kato, and H. Ohtake. “Isolation and Characterization of Chemotaxis Mutants and Genes of Pseudomonas Aeruginosa.” J Bacteriol 177, no. 4 (1995): 948-52. (PDF)

O’Toole, G. A., L. A. Pratt, P. I. Watnick, D. K. Newman, V. B. Weaver, and R. Kolter. “Genetic Approaches to Study of Biofilms.” Methods in Enzymology 310 (1999): 91-109.

Qiu, D., V. M. Eisinger, D. W. Rowen, and H. D. Yu. “Regulated Proteolysis Controls Mucoid Conversion in Pseudomonas Aeruginosa.” PNAS 104, no. 19 (2007): 8107-12. (PDF)

Rahme, L. G., E. J. Stevens, S. F. Wolfort, J. Shao, R. G. Tomkins, and F. M. Ausbel. “Common Virulence Factors for Bacterial Pathogenicity in Plants and Animals.” Science 268 (1995): 1899-1902.

Rashid, M. H., and A. Kornberg. “Inorganic Polyphosphate is Needed for Swimming, Swarming, and Twitching Motilities of Pseudomonas Aeruginosa.” PNAS 97, no. 9 (2000): 4885-90. (PDF)

Saltikov, C. W., and D. K. Newman. “Genetic Identification of a Respiratory Arsenate Reductase.” PNAS 100, no. 19 (2003): 10983-88. (PDF)

Shanks, R. M. Q., N. C. Caiazza, S. M. Hinsa, C. M. Toutain, and G. A. O’Toole. “Saccharomyces Cerevisiae-Based Molecular Tool Kit for Manipulation of Genes from Gram-Negative Bacteria.” Appl Environ Microbiol 72, no. 7 (2006): 5027-5036.

Silver, S., P. Johnseine, E. Whitney, and D. Clark. “Manganese-Resistant Mutants of Escherichia coli: Physiological and Genetic Studies.” J Bacteriol 110, no. 1 (1972): 186-95. (PDF - 1.3MB)

Smith, E. E., et al. “Genetic Adaptation by Pseudomonas Aeruginosa to the Airways of Cystic Fibrosis Patients.” PNAS 103, no. 22 (2006): 8487-92.

Vasil, M. L. “How We Learnt About Iron Acquisition in Pseudomonas Aeruginosa: A Series of Very Fortunate Events.” Biometals 20 (2007): 587-601.

Wiehlmann, L., et al. “Population Structure of Pseudomonas Aeruginosa.” PNAS 104, no. 19 (2007): 8101-6. (PDF - 1.5MB)

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