Labs | Experimental Microbial Genetics | Biology | MIT OpenCourseWare

Course Info

Instructors

Departments

Biology

As Taught In

Fall 2008

Level

Undergraduate

Topics

Science
- Biology
  - Genetics
  - Microbiology

Learning Resource Types

Problem Sets

Projects

Written Assignments

Download Course

Labs

Agarose Gel Electrophoresis and DNA Band Excision

Archiving the tools you make this term

Biofilm Assay

CAS Agar for Pseudomonas

Characterization of phenazines in Pseudomonas

Dephosphorylating with CIP and Ligation Reactions

Digital Optical Microscopy

Experimental Design and Interpretation, Technical Skill, Quality of Effort, Lab Notebook and Lab Citizenship

Generating and screening for mutants in Fur

List of common primers

Motility Assays

Mucoid Colony Assay

PCR with the Platinum PCR SuperMix High Fidelity

Plasmid DNA Purification Using the QIAprep Spin Miniprep Kit and a Microcentrifuge

Plasmid or Cosmid DNA Purification Using HiSpeed Plasmid Midi and Maxi Kits

Primer Design Guidelines for PCR Reactions

Project List

Protocol for spot cfu/ml counts

Pseudomonas Electroporations

QIAquick Gel Extraction Kit Protocol

QIAquick PCR & Enzyme Purification Kit Protocol

Restriction digests

Results from phenazine screen on tryptone plates

UV spectrophotometric measurement of DNA concentration and purity

Care and Handling of PA01 fur mutants

Transforming DH5a-T1R Chemically Competent Cells

pT9

pUCP18 sequence

pMQ64 map

pMQ64 sequence

Course Info

Instructors

Departments

Biology

As Taught In

Fall 2008

Level

Undergraduate

Topics

Science
- Biology
  - Genetics
  - Microbiology

Learning Resource Types

Problem Sets

Projects

Written Assignments

Download Course

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