7.15 | Spring 2015 | Undergraduate

Experimental Molecular Genetics

Calendar

SES # ACTIVITIES AND KEY DATES  
Week 1  
1

  • Safety Training
  • Introduction to the Course
  • C. Elegans Life Cycle, Anatomy and Behavior
  • Lab Specific Safety Walk Through
  • C. Elegans Maintenance and Manipulation Lab
  • Genetic Linkage and SNP Mapping

 
2

  • Forward and Reverse Genetics: An Overview of Classical Genetic Methods
  • C. Elegans Mutants and WormBase Workshop
  • Genetic Linkage and SNP Mapping
  • Using RNAi to Knockdown Gene Function

 
3

  • Laboratory Notebook: A Guide to Proper Management
  • Overview of Gibson Assembly Method for Molecular Cloning and Primer Design
  • Gibson Assembly
  • Using RNAi to Knockdown Gene Function

 
4

  • Workshop on NCBI Databases (MIT Libraries)
  • Genetic Linkage and SNP Mapping
  • Using RNAi to Knockdown Gene Function
  • Chemotaxis Assay

 
5

  • Genetic Linkage and SNP Mapping
  • PCR and Agarose Gel Electrophoresis Review
  • Gibson Assembly

 
Week 2  
6

  • Science Communication
  • Gibson Assembly
  • Using RNAi to Knockdown Gene Function
  • Collect Worms for RNA Extraction

 
7

  • Gibson Assembly
  • Using RNAi to Knockdown Gene Function
  • cDNA Synthesis

 
8

  • Gibson Assembly
  • Using RNAi to Knockdown Gene Function
  • Genetic Linkage and SNP Mapping

 
9

  • Genetic Linkage and SNP Mapping
  • Using RNAi to Knockdown Gene Function
  • Gibson Assembly

 
10 Begin work on individual projects  
Week 3: From this point forward, each student takes on their own individual project for the remainder of the semester  
11 Individual Student Project Work  
12 Individual Student Project Work  
13 Journal Club 1  
14 Individual Student Project Work  
15 Group Meeting: Research Proposal Discussion  
Week 4  
16 Individual Student Project Work  
17 Draft of Research Proposal Due  
18 Journal Club 2  
19 Individual Student Project Work  
20 Group Meeting  
Week 5
21 Individual Student Project Work  
22 Research Proposal Due  
23 Individual Student Project Work  
23 Individual Student Project Work  
24 Individual Student Project Work  
25 Group Meeting  
Week 6  
26 Individual Student Project Work  
27 Individual Student Project Work  
28 Journal Club 3  
29 Individual Student Project Work  
30 Group Meeting  
Week 7  
31 Individual Student Project Work  
32 Mini-review Due  
33 Individual Student Project Work  
34 Individual Student Project Work  
35 Group Meeting  
Week 8  
36 Individual Student Project Work  
37 Draft of Introduction Due  
38 Individual Student Project Work  
39 Progress Research Presentation  
40 Individual Student Project Work  
Week 9  
41 Midterm Evaluation Meetings  
42 Materials and Methods Due  
43 Journal Club 4  
44 Individual Student Project Work  
45 Group Meeting  
Week 10  
46 Individual Student Project Work  
47 Figures and Results Due  
48 Journal Club 5  
49 Individual Student Project Work  
50 Group Meeting  
Week 11  
51 Individual Student Project Work  
52 Individual Student Project Work  
53 Journal Club 6  
54 Individual Student Project Work  
55 Individual Student Project Work  
Week 12  
56 Individual Student Project Work  
57 Title, Abstract and Discussion Due  
58 Journal Club 7  
59 Individual Student Project Work  
60 Group Meeting  
Week 13  
61 Individual Student Project Work  
62 Final Presentation Rehearsals  
63 Journal Club 8  
64  Individual Student Project Work  
65 Group Meeting  
Week 14  
66 Individual Student Project Work  
67 Individual Student Project Work  
68 Final Oral Presentations  
69 Final Paper Due  
70 Individual Student Project Work  

Course Info

Departments
As Taught In
Spring 2015
Learning Resource Types
Presentation Assignments
Written Assignments