7.15 | Spring 2015 | Undergraduate

Experimental Molecular Genetics


The assigned readings below are discussed at the journal club meetings during the semester. Supplemental papers are listed at the bottom.

Journal Club 1, Ses 13

Main Paper

Sengupta, P., J. H. Chou, et al. “odr–10 Encodes a Seven Transmembrane Domain Olfactory Receptor Required for Responses to the Odorant Diacetyl.” Cell 84, no. 6 (1996): 899–909.

Additional Reading

Bargmann, C. I., E. Hartwieg, et al. “Odorant–selective Genes and Neurons Mediate Olfaction in C. elegans.” Cell 74, no. 3 (1993): 515–27.

Troemel, E. R., B. E. Kimmel, et al. “Reprogramming Chemotaxis Responses: Sensory Neurons Define Olfactory Preferences in C. elegans.” Cell 91, no. 2 (1997): 161–9.

Journal Club 2, Ses 18

Main Paper

Cabreiro, F., C. Au, et al. “Metformin Retards Aging in C. elegans by Altering Microbial Folate and Methionine Metabolism.” Cell 153, no. 1 (2013): 228–39.

Additional Readings

Pernicova, I., and M. Korbonits. “Metforminmode of Action and Clinical Implications for Diabetes and Cancer.” Nature Reviews Endocrinology 10, no. 3 (2014): 143–56.

Clemente, J. C., L. K. Ursell, et al. “The Impact of the Gut Microbiota on Human Health: An Integrative View.” Cell 148, no. 6 (2012): 1258–70.

Journal Club 3, Ses 28

Main Paper

Meisel, J. D., O. Panda, et al. “Chemosensation of Bacterial Secondary Metabolites Modulates Neuroendocrine Signaling and Behavior of C. elegans.” Cell 159, no. 2 (2014): 267–80.

Additional Readings

Price–Whelan, A., L. E. Dietrich, et al. “Rethinking ‘Secondary’ Metabolism: Physiological Roles for Phenazine Antibiotics.” Nature Chemical Biology 2, no. 2 (2006): 71–8.

Prasad, Brinda C., and Randall R. Reed. “Chemosensation: Molecular Mechanisms in Worms and Mammals.” Trends in Genetics 15, no. 4 (1999): 150–3.

Journal Club 4, Ses 43

Main Paper

Ghosh, R., E. C. Andersen, et al. “Natural Variation in a Chloride Channel Subunit Confers Avermectin Resistance in C. elegans.” Science 335, no. 6068 (2012): 574–8.

Additional Readings

Andersen, E. C., J. P. Gerke, et al. “Chromosome–scale Selective Sweeps Shape Caenorhabditis Elegans Genomic Diversity.” Nature Genetics 44, no. 3 (2012): 285–90.

Manolio, T. A., et al. “Finding the Missing Heritability of Complex Diseases.” Nature 461, no. 7265 (2009): 747–53.

Journal Club 5, Ses 48

Main Paper

Costanzo, M., A. Baryshnikova, et al. “The Genetic Landscape of a Cell.” Science 327, no. 5964 (2010): 425–31.

Additional Reading

J. L., HartmanIV, Garvik B., et al. “Principles for the Buffering of Genetic Variation.” Science 291, no. 5506 (2001): 1001–4.

Chan, D. A., and A. J. Giaccia. “Harnessing Synthetic Lethal Interactions in Anticancer Drug Discovery.” Nature Reviews Drug Discovery 10, no. 5 (2011): 351–64.

Journal Club 6, Ses 53

Main Paper

Wang, T., J. J. Wei, et al. “Genetic Screens in Human Cells Using the CRISPR–Cas9 System.” Science 343, no. 6166 (2014): 80–4.

Additional Reading

Cong, L, et al. “Multiplex Genome Engineering Using CRISPR/Cas Systems.” Science 339, no. 6121 (2013): 819–23.

Barrangou, R., and L. A. Marraffini. “CRISPR–Cas Systems: Prokaryotes Upgrade to Adaptive Immunity.” Molecular Cell 54, no. 2 (2014): 234–44.

Journal Club 7, Ses 58

Main Paper

Weinreich, D. M., N. F. Delaney, et al . “[Darwinian Evolution Can Follow Only Very Few Mutational Paths to Fitter Proteins](http://dx.doi.org/ 10.1126/science.1123539).” Science 312, no. 5770 (2006): 111–4.

Additional Reading

Tokuriki, N., and D. S. Tawfik. “Protein Dynamism and Evolvability.” Science 324, no. 5924 (2009): 203–7.

Journal Club 8, Ses 63

Main Paper

Holmes, D. L., A. K. Lancaster, et al. “Heritable Remodeling of Yeast Multicellularity by an Environmentally Responsive Prion.” Cell 153, no. 1 (2013): 153–65.

Additional Reading

Halfmann, R., D. F. Jarosz. “Prions are a Common Mechanism for Phenotypic Inheritance in Wild Yeasts.” Nature 482 (2012): 363–8.

Soto, C. “Transmissible Proteins: Expanding the Prion Heresy.” Cell 149, no. 5 (2012): 968–77.

Supplemental Papers

Jones, A. K., S. D. Buckingham, et al. “Chemistry-to-Gene Screens in Caenorhabditis Elegans.” Nature Reviews Drug Discovery 4, no. 4 (2005): 321–30.

Jorgensen, E. M., and S. E. Mango. “The Art and Design of Genetic Screens: Caenorhabditis Elegans.” Nature Reviews Genetics 3, no. 5 (2002): 356–69.

Course Info

As Taught In
Spring 2015
Learning Resource Types
Presentation Assignments
Written Assignments