Experimental Molecular Genetics

Calendar

SES #	ACTIVITIES AND KEY DATES
Week 1
1	Safety Training Introduction to the Course C. Elegans Life Cycle, Anatomy and Behavior Lab Specific Safety Walk Through C. Elegans Maintenance and Manipulation Lab Genetic Linkage and SNP Mapping
2	Forward and Reverse Genetics: An Overview of Classical Genetic Methods C. Elegans Mutants and WormBase Workshop Genetic Linkage and SNP Mapping Using RNAi to Knockdown Gene Function
3	Laboratory Notebook: A Guide to Proper Management Overview of Gibson Assembly Method for Molecular Cloning and Primer Design Gibson Assembly Using RNAi to Knockdown Gene Function
4	Workshop on NCBI Databases (MIT Libraries) Genetic Linkage and SNP Mapping Using RNAi to Knockdown Gene Function Chemotaxis Assay
5	Genetic Linkage and SNP Mapping PCR and Agarose Gel Electrophoresis Review Gibson Assembly
Week 2
6	Science Communication Gibson Assembly Using RNAi to Knockdown Gene Function Collect Worms for RNA Extraction
7	Gibson Assembly Using RNAi to Knockdown Gene Function cDNA Synthesis
8	Gibson Assembly Using RNAi to Knockdown Gene Function Genetic Linkage and SNP Mapping
9	Genetic Linkage and SNP Mapping Using RNAi to Knockdown Gene Function Gibson Assembly
10	Begin work on individual projects
Week 3: From this point forward, each student takes on their own individual project for the remainder of the semester
11	Individual Student Project Work
12	Individual Student Project Work
13	Journal Club 1
14	Individual Student Project Work
15	Group Meeting: Research Proposal Discussion
Week 4
16	Individual Student Project Work
17	Draft of Research Proposal Due
18	Journal Club 2
19	Individual Student Project Work
20	Group Meeting
Week 5
21	Individual Student Project Work
22	Research Proposal Due
23	Individual Student Project Work
23	Individual Student Project Work
24	Individual Student Project Work
25	Group Meeting
Week 6
26	Individual Student Project Work
27	Individual Student Project Work
28	Journal Club 3
29	Individual Student Project Work
30	Group Meeting
Week 7
31	Individual Student Project Work
32	Mini-review Due
33	Individual Student Project Work
34	Individual Student Project Work
35	Group Meeting
Week 8
36	Individual Student Project Work
37	Draft of Introduction Due
38	Individual Student Project Work
39	Progress Research Presentation
40	Individual Student Project Work
Week 9
41	Midterm Evaluation Meetings
42	Materials and Methods Due
43	Journal Club 4
44	Individual Student Project Work
45	Group Meeting
Week 10
46	Individual Student Project Work
47	Figures and Results Due
48	Journal Club 5
49	Individual Student Project Work
50	Group Meeting
Week 11
51	Individual Student Project Work
52	Individual Student Project Work
53	Journal Club 6
54	Individual Student Project Work
55	Individual Student Project Work
Week 12
56	Individual Student Project Work
57	Title, Abstract and Discussion Due
58	Journal Club 7
59	Individual Student Project Work
60	Group Meeting
Week 13
61	Individual Student Project Work
62	Final Presentation Rehearsals
63	Journal Club 8
64	Individual Student Project Work
65	Group Meeting
Week 14
66	Individual Student Project Work
67	Individual Student Project Work
68	Final Oral Presentations
69	Final Paper Due
70	Individual Student Project Work