All parts are specified at the Registry of Standard Biological Parts. Refine the parts there as well as here.
GENETIC ELEMENT | PROMOTER | RBS | CODING |
---|---|---|---|
Start synthesis with | |||
gII |
BBa_M13102 (need 5’ UTR?) |
BBa_M13502 |
BBa_M13002’ (modified to remove gene 10 promoter) |
gX |
BBa_M13110 (need 5’ UTR?) |
BBa_M13510 |
BBa_M13010’ (modified to remove gene 5 promoter) |
gV |
BBa_M13105 (need 5’ UTR?) |
BBa_M13505 | BBa_M13005 |
gVII | BBa_M13507 |
BBa_M13007’ (modified to remove overlap with gene 9 dwnstm) |
|
gIX | BBa_M13509 |
BBa_M13009’ (modified to remove overlap with gene 8 dwnstm) |
|
gVIII |
BBa_M13108 (need 5’ UTR?) |
BBa_M13508 | BBa_M13008 |
Transcriptional terminator (if M13KO7 part, then need to modify to remove gene 3 promoter) | |||
gIII | BBa_M13103 | BBa_M13503 |
BBa_M13003’ (modified to remove gene 6 promoter, change GTG start?) |
gVI | BBa_M13106 | BBa_M13506 |
BBa_M13006’ (modified to remove gene 1 promoter) |
gI | BBa_M13101 | BBa_M13501 |
BBa_M13001’ (modified to remove gene 11 RBS, gene 4 promoter, RBS, start) |
gXI | BBa_M13511 |
BBa_M13011’ (modified to remove gene 4 promoter, RBS, start) |
|
gIV |
BBa_M13104 (need 5’ UTR?) |
BBa_M13504 | BBa_M13004' |
M13KO7 ori/KanR/p15a ori | |||
End synthesis |