Lab Day 1
- Part 1.1: Digestion of mTn3 transposon library with NotI
- Part 1.2: Pour agarose gel
- Part 1.3: Prepare 1X TAE, 10X TE, and 10X LiAc
Lab Day 2
- Part 1.4: Gel analysis of mTn3 plasmid library DNA digest
- Part 1.5: Transformation of mTn3 library into yeast
Lab Day 3
- Part 1.6: Replica-plating onto α-factor plates
- Part 1.7: Prepare YPD liquid media
Lab Day 4
- Part 1.8: Identifying α-factor-resistant candidates
- Part 1.9: Streaking α-factor-resistant candidates onto new plates
