7.003 | Spring 2022 | Undergraduate

Applied Molecular Biology Lab

Experiment 3, Lab Day 7

Parts Pages in Lab Manual
Part 3.1: Yeast transformation of the pRSQ2 recovery plasmid 51–54

Lab Day 7 In-Lab Questions (PDF)

Supporting Files

Note: The (.dna) files used in this course contain DNA sequence data that are necessary to complete the in-lab questions. SnapGene is a molecular biology software application that is used to view and work with these files and is available to try for free

pRSQ2-URA3 Plasmid (.dna)

mTn3 Transposon (.dna)

(Source: National Library of Medicine, accessed in Fall 2022.)

Pre-Lab and Post-Lab Instructions

Day 7 Pre-Lab Tips and Reminders

What is your Aim for Part 3.1?

Day 7 Post-Lab Notebook Tips and Reminders

There is no post-lab for Day 7 because you did not obtain any results today, but you should make a note of any deviations you made from the lab manual protocols for your own records.

Course Info

Spring 2022
Activity Assignments
Laboratory Assignments
Problem Sets
Written Assignments