Lab Day 7
- Part 3.1: Yeast transformation of the pRSQ2 recovery plasmid
Lab Day 8
- Part 3.2: Genomic DNA prep from pRSQ2 transformants
Lab Day 9
- Part 3.3: Complete genomic DNA prep from pRSQ2 transformants
- Part 3.4: Restriction enzyme digest of the genomic DNA
- Part 3.5: Pour agarose gel
Lab Day 10
- Part 3.6: Gel analysis of digested genomic DNA
- Part 3.7: Ligation of the genomic DNA digest
Lab Day 11
- Part 3.8: Purification of ligation reactions
- Part 3.9: Preparation of inverse PCR reactions
- Part 3.10: Pour agarose gel
Lab Day 12
- Part 3.11: Gel analysis of inverse PCR products
- Part 3.12: Purification of inverse PCR products
- Part 3.13: Send out inverse PCR products for sequencing
Lab Day 13
- Part 3.14: BLAST analysis of inverse PCR sequencing results
- Part 3.15: Re- streaking the mutant strains
