7.003 | Spring 2022 | Undergraduate

Applied Molecular Biology Lab

Experiment 3, Lab Day 10

Parts Pages in Lab Manual
Part 3.6: Gel analysis of digested genomic DNA 63–64
Part 3.7: Ligation of the genomic DNA digest 64–66

Day 10 In-Lab Questions (PDF)

Pre-Lab and Post-Lab Instructions

Day 10 Pre-Lab Tips and Reminders

Remember to include Aims for Parts 3.6 and 3.7 today.

Day 10 Post-Lab Tips and Reminders

  • Be sure to label the gel photo such that it’s clear what is loaded in each lane and what the sizes of all ladder bands are.
  • You should discuss each lane of your gel (lanes/samples with similar overall results can be discussed together as a group if you want).
    • What was the expected overall result for each lane? Why?
    • What can you conclude based on whether or not you saw the expected result for each lane? Explain your reasoning. If you did not get the expected results, what might be a possible reason to explain the difference?

Course Info

Departments
As Taught In
Spring 2022
Learning Resource Types
Labs
Activity Assignments
Problem Sets
Written Assignments