7.003 | Spring 2022 | Undergraduate

Applied Molecular Biology Lab

Experiment 3, Lab Day 7

Parts Pages in Lab Manual
Part 3.1: Yeast transformation of the pRSQ2 recovery plasmid 51–54

Lab Day 7 In-Lab Questions (PDF)

Supporting Files

Note: The (.dna) files used in this course contain DNA sequence data that are necessary to complete the in-lab questions. SnapGene is a molecular biology software application that is used to view and work with these files and is available to try for free

pRSQ2-URA3 Plasmid (.dna)

mTn3 Transposon (.dna)

(Source: National Library of Medicine, accessed in Fall 2022.)

Pre-Lab and Post-Lab Instructions

Day 7 Pre-Lab Tips and Reminders

What is your Aim for Part 3.1?

Day 7 Post-Lab Notebook Tips and Reminders

There is no post-lab for Day 7 because you did not obtain any results today, but you should make a note of any deviations you made from the lab manual protocols for your own records.

Course Info

Departments
As Taught In
Spring 2022
Learning Resource Types
Labs
Activity Assignments
Problem Sets
Written Assignments