| Parts | Pages in Lab Manual |
|---|---|
| Part 4.1: Isolation of total RNA from WT and mutant yeast | 82–87 |
| Part 4.2: Pour agarose gel | 87–88 |
Pre-Lab and Post-Lab Instructions
Day 14 Pre-Lab Notebook Tips and Reminders
Remember to include Aims for all parts (Parts 4.1–4.2).
Day 14 Post-Lab Notebook Tips and Reminders
- Include all your spectrophotometer OD600 readings and calculations for determining cell concentration and volume of cell cultures to use.
- Include all spectrophotometer RNA concentration measurements/results.
- How successful do you think your overall RNA prep was, based on your spectrophotometer results? What can you conclude from the A260/A280 ratios?
