Week 1:
Tu:
Lecture 1: Genetic Screens, Model Organisms, Transposon Mutagenesis
Lab: Class Logistics
Th:
Lab: Training Lab
Week 2:
Tu:
Lecture 2: Yeast Mating Pathway
Lab Day 1: Notl Library Digest; Pour Gel; Make Buffers
Day 1 Pre-Lab due
Th:
Lab Day 2: Run Notl Digests on Gel; Transform Library PPY295
Day 1 Post-Lab and Day 2 Pre-Lab due
Week 3:
Tu:
Lecture 3: Orthogonal Assays
Lab Day 3: RP Transformants to α-Factor; Make Media
Day 2 Post-Lab and Day 3 Pre-Lab due
Th:
Lab Day 4: Streak α-Factor-Resistant Mutants
Day 3 Post Lab and Day 4 Pre-Lab due
Week 4:
Tu:
[No Lecture]
[No Lab]
Th:
Lab Day 5: Perform Shmoo Assay; Setup Halo; Mating; Sporulation Assay
Day 4 Post-Lab and Day 5 Pre-Lab due
Week 5:
Tu:
Lecture 4: Plasmid Recovery
Lab Day 6: Analyze Halo; Mating; Sporulation Assay
Day 5 Post-Lab and Day 6 Pre-Lab due
Th:
Lab Day 7: Transform pRSQ2
Day 6 Post-Lab and Day 7 Pre-Lab due
Fr:
Days 1-4 Post-Lab Regrades (optional)
Week 6:
Tu:
Lecture 5: DNA Sequencing Methods & Analysis
Lab Day 8: gDNA Prep, Part 1
Day 8 Pre-Lab due
Th:
Lab Day 9: gDNA Prep, Part 2; EcoRI gDNA Digest; Pour Gel
Day 8 Post-Lab and Day 9 Pre-Lab due
Week 7:
Tu:
Lecture 6: Gene Expression Analysis
Lab Day 10: Run EcoRI Digest Gel; Setup Ligations
Day 9 Post-Lab and Day 10 Pre-Lab due
Th:
Lab Day 11: Purify Ligations; Setup iPCR; Pour Gel
Day 10 Post-Lab and Day 11 Pre-Lab due
Fr:
Problem Set #1 due
Days 5-8 Post-Lab Regrades (optional)
Week 8:
Spring Break: No Lecture or Lab
Week 9:
Tu:
Lecture 7: Next-Gen Sequencing Applications
Lab Day 12: Run iPCR Gel; Purify iPCR; Send iPCR for Sequencing
Day 12 Pre-Lab due
Th:
Lab Day 13: BLAST Analysis
Day 12 Post-Lab and Day 13 Pre-Lab due
Fr:
Problem Set #1 Regrade due
Week 10:
Tu:
Lecture 8: Genetic Engineering Techniques
Lab Day 14: RNA Prep; Pour Gel
Day 13 Post-Lab and Day 14 Pre-Lab due
Th:
Lab Day 15: Run RNA Gel; Make cDNA
Day 14 Post-Lab and Day 15 Pre-Lab due
Fr:
Days 9-12 Post-Lab Regrades (optional)
Week 11:
Tu:
Lecture 9: Directed Evolution and Yeast Surface Display
Lab Day 16: Setup cDNA Test PCR; Pour Gel
Day 15 Post-Lab and Day 16 Pre-Lab due
Th:
Lab Day 17: Run cDNA PCR Gel; Setup qPCR Plate
Day 17 Pre-Lab due
Week 12:
Tu:
Lecture 10: COVID Diagnostics
Lab Day 18: Analyze qPCR Results
Day 17 Post-Lab and Day 18 Pre-Lab due
Th:
Lab Day 19: COVID Target Design
Day 18 Post-Lab and Day 19 Pre-Lab due
Fr:
Days 13-15 Post-Lab Regrades (optional)
Week 13:
Tu:
[No Lecture]
Lab Day 20: Magnetic Bead Selection
Day 19 Post-Lab and Day 20 Pre-Lab due
W:
Problem Set #2 due
Th:
Lab Day 21: Optimize Bead Selection; FACS Analysis
Day 20 Post-Lab and Day 21 Pre-Lab due
Week 14:
Tu:
[No Lecture]
Lab Day 22: Analyze Bead Selection Results
Day 21 Post-Lab and Day 22 Pre-Lab due
Th:
Lab Day 23: Clonal Analysis
Day 22 Post-Lab and Day 23 Pre-Lab due
Day 23 Post-Lab due by 11:59 PM
Fr:
Days 17-21 Post-Lab Regrades (optional)
Problem Set #2 Regrade (optional)
