7.01SC | Fall 2011 | Undergraduate

Fundamentals of Biology

Recombinant DNA

Development of Recombinant DNA

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Session Overview

RecombDNA_sess1.jpg

This session will outline why recombinant DNA technologies were developed and how they are used today in many different scientific arenas.

Learning Objectives

  • To understand some of the applications of recombinant DNA technologies.
  • To have an overview of the steps involved in a basic cloning strategy.

Session Activities

Lecture Video

Watch the lecture video excerpt

Check Yourself

Question 1

Arrange the following steps in the correct order 1. Screen the bacterial colonies 2. Transform DNA into host bacterial cells 3. Cut DNA of interest 4. Select bacterial host cells that have been transformed 5. Paste DNA of interest into vectors

Session Activities

Lecture Video

Watch the lecture video excerpt

Further Study

Suggested topics for further study in an introductory-level Biology textbook

  • applications of recombinant DNA technologies

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